Antagonistic effect of inositol pentakisphosphate on inositol triphosphate receptors

Pei Jung Lu, Woan Ru Shieh, Ching Shih Chen

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We present evidence that inositol 1,3,4,5,6-pentakisphosphate acts as an antagonist of inositol triphosphate receptors in vitro by dampening the activity of Ca2+-mobilizing inositol phosphates. This antagonism bears physiological significance as cells maintain substantial quantities of this phosphoinositol in the cytosol, especially during cell differentiation or proliferation. Over a concentration range from 30 to 150 μM, this pentakisphosphate causes a two- to five-fold increase in the EC50 values of inositol 1,4,5-trisphosphate and other active phosphoinositols in effecting Ca2+ efflux from brain microsomes. This specific inhibition results from competitive receptor binding, and is not noted with inositol hexaphosphate or related metabolites at comparable concentrations. Thus, inositol 1,3,4,5,6-pentakisphosphate in concert with other allosteric modulators may constitute an integral component for the receptor regulation.

Original languageEnglish
Pages (from-to)637-642
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume220
Issue number3
DOIs
Publication statusPublished - 1996 Mar 27

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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