Antagonizing TGF-β induced liver fibrosis by a retinoic acid derivative through regulation of ROS and calcium influx

Kun Lin Yang, Wen Teng Chang, Chia Chang Chuang, Kuo Chen Hung, Eric I.C. Li

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Transforming growth factor-beta1 (TGF-β1) mediates the regulation of extracellular matrix via reactive oxygen species (ROS) and calcium influx, both are activators of hepatic stellate cells (HSC) which play a critical role in hepatic fibrogenesis. Hence one can use ROS assay as the main screening tool for molecules that might antagonize the process of liver fibrosis. A retinoic acid derivative isolated from the mycelium of Phellinus linteus that down-regulates ROS generation and calcium influx in HSC-T6 cells was thus obtained in our screening process. The retinoic acid derivative also reverses an early liver fibrosis, as assayed by liver contents of hydroxyproline, α-smooth muscle actin (α-SMA), and collagen 1A2, in an early liver fibrosis model we established previously where an inducible expression vector containing a TGF-β gene was hydrodynamically transferred into a testing animal. Retinoic acid derivative thus acts both in vitro and in vivo to prevent liver fibrosis at an early phase.

Original languageEnglish
Pages (from-to)484-489
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume365
Issue number3
DOIs
Publication statusPublished - 2008 Jan 18

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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