Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

Bing Hung Chen, Mei Huei Hung, Jeff Yi Fu Chen, Hsueh Wei Chang, Meng Lung Yu, Lei Wan, Fuu Jen Tsai, Tzu Pin Wang, Tzu Fun Fu, Chien Chih Chiu

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca 2+ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.

Original languageEnglish
Pages (from-to)968-974
Number of pages7
JournalFood Chemistry
Issue number2
Publication statusPublished - 2012 May 15

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science

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