Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

Bing Hung Chen; Mei Huei Hung; Jeff Yi Fu Chen; Hsueh Wei Chang; Meng Lung Yu; Lei Wan; Fuu Jen Tsai; Tzu Pin Wang; Tzu Fun Fu; Chien Chih Chiu

doi:10.1016/j.foodchem.2011.11.079

Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

Bing Hung Chen, Mei Huei Hung, Jeff Yi Fu Chen, Hsueh Wei Chang, Meng Lung Yu, Lei Wan, Fuu Jen Tsai, Tzu Pin Wang, Tzu Fun Fu, Chien Chih Chiu

Department of Medical Laboratory Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca ²⁺ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.

Original language	English
Pages (from-to)	968-974
Number of pages	7
Journal	Food Chemistry
Volume	132
Issue number	2
DOIs	https://doi.org/10.1016/j.foodchem.2011.11.079
Publication status	Published - 2012 May 15

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2011.11.079

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title = "Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells",

abstract = "Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca 2+ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.",

author = "Chen, {Bing Hung} and Hung, {Mei Huei} and Chen, {Jeff Yi Fu} and Chang, {Hsueh Wei} and Yu, {Meng Lung} and Lei Wan and Tsai, {Fuu Jen} and Wang, {Tzu Pin} and Fu, {Tzu Fun} and Chiu, {Chien Chih}",

note = "Funding Information: This work was partly supported by Grants from the National Science Council , Taiwan ( NSC95-2320-B-037-045 ), The Department of Health , Taiwan ( DOH100-TD-C-111-002 ) and Kaohsiung Medical University ( KMU-M098007 and KMU-EM-99-1.4 ).",

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AU - Chen, Bing Hung

AU - Hung, Mei Huei

AU - Chen, Jeff Yi Fu

AU - Chang, Hsueh Wei

AU - Yu, Meng Lung

AU - Wan, Lei

AU - Tsai, Fuu Jen

AU - Wang, Tzu Pin

AU - Fu, Tzu Fun

AU - Chiu, Chien Chih

N1 - Funding Information: This work was partly supported by Grants from the National Science Council , Taiwan ( NSC95-2320-B-037-045 ), The Department of Health , Taiwan ( DOH100-TD-C-111-002 ) and Kaohsiung Medical University ( KMU-M098007 and KMU-EM-99-1.4 ).

PY - 2012/5/15

Y1 - 2012/5/15

N2 - Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca 2+ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.

AB - Grapeseed extract (GSE) is a rich source of natural phenolic compounds and possesses various pharmacological activities, including antioxidant, anticarcinogenic and anti-inflammatory properties. However, effects of GSE on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of GSE on activation and degranulation of RBL-2H3 mast cells were investigated. GSE pretreatments (20-100 μg/ml) reduced IgE-antigen mediated release of β-hexosaminidase and histamine in RBL-2H3 cells. Additionally, GSE reversibly inhibited expression of FcRI on RBL-2H3 cells. GSE treatments caused a significant elevation of intracellular cAMP levels, whereas the Ca 2+ influx upon antigen stimulation was inhibited. Suppression on FcRI expression together with decreased calcium uptake and increased cAMP level might be involved in attenuated degranulation of mast cells by GSE treatment. Our results suggest a possible pharmaceutical application of GSE in treating type I allergic diseases.

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Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells

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