Anti-Hepatitis B surface Antigen monoclonal antibody IgM production in suspension and immobilized cell bioreactors

Chi Wai Kwong; Jih Han Hsieh; Mei-Jywan Syu; Shan Shan Chou

doi:10.1007/BF01089467

Anti-Hepatitis B surface Antigen monoclonal antibody IgM production in suspension and immobilized cell bioreactors

Chi Wai Kwong, Jih Han Hsieh, Mei-Jywan Syu, Shan Shan Chou

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We have cultivated a hybridoma cell-line H505AC in suspension and alginate immobilized cell bioreactors to produce anti-Hepatitis B surface Antigen (anti-HBsAg) monoclonal antibody IgM. The specific IgM production rates correlate linearly with the specific glucose consumption rate in suspension culture with a maximum production rate of 300 μg/10⁶ cells/day. In alginate-cell immobilized airlift bioreactor, a total of 1143 milligrams IgM was produced in 9 days operation with a volumetric productivity 44.1 mg/day.L

Original language	English
Pages (from-to)	377-382
Number of pages	6
Journal	Biotechnology Letters
Volume	11
Issue number	6
DOIs	https://doi.org/10.1007/BF01089467
Publication status	Published - 1989 Jun 1

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF01089467

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title = "Anti-Hepatitis B surface Antigen monoclonal antibody IgM production in suspension and immobilized cell bioreactors",

abstract = "We have cultivated a hybridoma cell-line H505AC in suspension and alginate immobilized cell bioreactors to produce anti-Hepatitis B surface Antigen (anti-HBsAg) monoclonal antibody IgM. The specific IgM production rates correlate linearly with the specific glucose consumption rate in suspension culture with a maximum production rate of 300 μg/106 cells/day. In alginate-cell immobilized airlift bioreactor, a total of 1143 milligrams IgM was produced in 9 days operation with a volumetric productivity 44.1 mg/day.L",

author = "Kwong, {Chi Wai} and Hsieh, {Jih Han} and Mei-Jywan Syu and Chou, {Shan Shan}",

year = "1989",

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doi = "10.1007/BF01089467",

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T1 - Anti-Hepatitis B surface Antigen monoclonal antibody IgM production in suspension and immobilized cell bioreactors

AU - Kwong, Chi Wai

AU - Hsieh, Jih Han

AU - Syu, Mei-Jywan

AU - Chou, Shan Shan

PY - 1989/6/1

Y1 - 1989/6/1

N2 - We have cultivated a hybridoma cell-line H505AC in suspension and alginate immobilized cell bioreactors to produce anti-Hepatitis B surface Antigen (anti-HBsAg) monoclonal antibody IgM. The specific IgM production rates correlate linearly with the specific glucose consumption rate in suspension culture with a maximum production rate of 300 μg/106 cells/day. In alginate-cell immobilized airlift bioreactor, a total of 1143 milligrams IgM was produced in 9 days operation with a volumetric productivity 44.1 mg/day.L

AB - We have cultivated a hybridoma cell-line H505AC in suspension and alginate immobilized cell bioreactors to produce anti-Hepatitis B surface Antigen (anti-HBsAg) monoclonal antibody IgM. The specific IgM production rates correlate linearly with the specific glucose consumption rate in suspension culture with a maximum production rate of 300 μg/106 cells/day. In alginate-cell immobilized airlift bioreactor, a total of 1143 milligrams IgM was produced in 9 days operation with a volumetric productivity 44.1 mg/day.L

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 6

ER -

Anti-Hepatitis B surface Antigen monoclonal antibody IgM production in suspension and immobilized cell bioreactors

Abstract

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