Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma

Yi Shu Chiu, Chung Hsi Hsing, Chien Feng Li, Chon Yee Lee, Yu Hsiang Hsu, Ming Shi Chang

Research output: Contribution to journalArticle

Abstract

Interleukin (IL)-20 is a proinflammatory cytokine involved in rheumatoid arthritis, atherosclerosis, and osteoporosis. However, the role of IL-20 in hepatocellular carcinoma (HCC) is unclear. We explored the function of IL-20 in HCC. Tumor tissue samples were analyzed the expression of IL-20 and cyclin D1 by using immunohistochemistry staining and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. To examine the role of anti-IL-20 monoclonal antibody (7E) in tumor growth, BALB/c mice was injected with ML-1 cells and treated with 7E. HCC tumor tissue expressed higher levels of IL-20 than did non-tumor tissue. High IL-20 expression in HCC was correlated with poor overall survival (relative risk:>3). IL-20 and cyclin D1 expression were also highly correlated in HCC patient specimens and 3 human HCC cell lines. IL-20 also increased cell proliferation and migration, and it regulated matrix metalloproteinase (MMP)-13, tumor necrosis factor (TNF)-α, cyclin D1, and p21WAF1 expression in ML-1 cells. 7E attenuated tumor growth in mice inoculated with ML-1 cells. The expression of cyclin D1, TNF-α, MMP-9, and vascular endothelial growth factor was significantly inhibited after 7E treatment. The findings of this study suggest that IL-20 plays a role in the tumor progression of HCC.

Original languageEnglish
Article number17609
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Hepatocellular Carcinoma
Monoclonal Antibodies
Growth
Cyclin D1
Neoplasms
Tumor Necrosis Factor-alpha
interleukin 20
Matrix Metalloproteinase 13
Matrix Metalloproteinase 9
Vascular Endothelial Growth Factor A
Osteoporosis
Cell Movement
Real-Time Polymerase Chain Reaction
Rheumatoid Arthritis
Atherosclerosis
Immunohistochemistry
Cell Proliferation
Staining and Labeling
Cytokines
Cell Line

All Science Journal Classification (ASJC) codes

  • General

Cite this

Chiu, Yi Shu ; Hsing, Chung Hsi ; Li, Chien Feng ; Lee, Chon Yee ; Hsu, Yu Hsiang ; Chang, Ming Shi. / Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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abstract = "Interleukin (IL)-20 is a proinflammatory cytokine involved in rheumatoid arthritis, atherosclerosis, and osteoporosis. However, the role of IL-20 in hepatocellular carcinoma (HCC) is unclear. We explored the function of IL-20 in HCC. Tumor tissue samples were analyzed the expression of IL-20 and cyclin D1 by using immunohistochemistry staining and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. To examine the role of anti-IL-20 monoclonal antibody (7E) in tumor growth, BALB/c mice was injected with ML-1 cells and treated with 7E. HCC tumor tissue expressed higher levels of IL-20 than did non-tumor tissue. High IL-20 expression in HCC was correlated with poor overall survival (relative risk:>3). IL-20 and cyclin D1 expression were also highly correlated in HCC patient specimens and 3 human HCC cell lines. IL-20 also increased cell proliferation and migration, and it regulated matrix metalloproteinase (MMP)-13, tumor necrosis factor (TNF)-α, cyclin D1, and p21WAF1 expression in ML-1 cells. 7E attenuated tumor growth in mice inoculated with ML-1 cells. The expression of cyclin D1, TNF-α, MMP-9, and vascular endothelial growth factor was significantly inhibited after 7E treatment. The findings of this study suggest that IL-20 plays a role in the tumor progression of HCC.",
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Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma. / Chiu, Yi Shu; Hsing, Chung Hsi; Li, Chien Feng; Lee, Chon Yee; Hsu, Yu Hsiang; Chang, Ming Shi.

In: Scientific reports, Vol. 7, No. 1, 17609, 01.12.2017.

Research output: Contribution to journalArticle

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