Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection

Yen Chung Lai, Yung Chun Chuang, Ching-Chuan Liu, Tzong-Shiann Ho, Yee-Shin Lin, Robert Anderson, Trai-Ming Yeh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging to develop. Based on the epitope recognized by the anti-NS1 monoclonal Ab (mAb) 33D2 which recognizes a conserved NS1 wing domain (NS1-WD) region but not host proteins, we synthesized a modified NS1-WD peptide to immunize mice. We found that both mAb 33D2 and modified NS1-WD peptide immune sera could induce complement-dependent lysis of dengue-infected but not un-infected cells in vitro. Furthermore, either active immunization with the modified NS1-WD peptide or passive transfer of mAb 33D2 efficiently protected mice against all serotypes of dengue virus infection. More importantly, dengue patients with more antibodies recognized the modified NS1-WD peptide had less severe disease. Thus, the modified NS1-WD peptide is a promising dengue vaccine candidate.

Original languageEnglish
Article number6975
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

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Dengue Virus
Virus Diseases
Antibodies
Proteins
Dengue
Peptides
Protein Domains
Dengue Vaccines
Vaccines
Molecular Mimicry
Culicidae
Immune Sera
Epitopes
Immunization
Vaccination

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging to develop. Based on the epitope recognized by the anti-NS1 monoclonal Ab (mAb) 33D2 which recognizes a conserved NS1 wing domain (NS1-WD) region but not host proteins, we synthesized a modified NS1-WD peptide to immunize mice. We found that both mAb 33D2 and modified NS1-WD peptide immune sera could induce complement-dependent lysis of dengue-infected but not un-infected cells in vitro. Furthermore, either active immunization with the modified NS1-WD peptide or passive transfer of mAb 33D2 efficiently protected mice against all serotypes of dengue virus infection. More importantly, dengue patients with more antibodies recognized the modified NS1-WD peptide had less severe disease. Thus, the modified NS1-WD peptide is a promising dengue vaccine candidate.",
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Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection. / Lai, Yen Chung; Chuang, Yung Chun; Liu, Ching-Chuan; Ho, Tzong-Shiann; Lin, Yee-Shin; Anderson, Robert; Yeh, Trai-Ming.

In: Scientific reports, Vol. 7, No. 1, 6975, 01.12.2017.

Research output: Contribution to journalArticle

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