Antibody production in Japanese eels, Anguilla japonica Temminck and Schlegel

H. W. Hung, C. F. Lo, C. C. Tseng, G. H. Kou

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Adult Japanese eels, Anguilla japonica Temminck and Schlegel, (200-250 g, 45-55 cm) were immunized by intramuscular injection with goat IgG. After 5 weeks, eel immunoglobulin (Ig) was purified using affinity chromatography. The purified eel Ig was used to immunize rabbits to produce anti-eel Ig antibody. The highest antibody ELISA value in eels was reached 3 weeks after initial immunization with goat IgG, and then gradually decreased. The antibody could still be detected at 140 days post-immunization. The optimal temperature for antibody production was 30°C. Freund's complete adjuvant and secondary immunization both increased antibody production in eels.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalJournal of Fish Diseases
Volume20
Issue number3
DOIs
Publication statusPublished - 1997 May

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • veterinary (miscalleneous)

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