TY - JOUR
T1 - Anticholinergic properties of brompheniramine, chlorpheniramine, and atropine in human nasal mucosa in vitro
AU - Fang, Sheen Yie
AU - Druce, Howard M.
AU - Baraniuk, James N.
PY - 1998/1/1
Y1 - 1998/1/1
N2 - Brompheniramine and chlorpheniramine have anticholinergic activities, but the relative potency of these effects has not been well defined. The anticholinergic properties of brompheniramine, chlorpheniramine, and atropine were assessed in an in vitro model of human nasal mucosal glandular secretion. Methacholine was used as a cholinergic agonist to stimulate glandular secretion of 7F10-mucin. These drugs (0.01-1000 μM) or vehicle (saline) were added to explant cultures with and without 100 μM methacholine. 7F10-mucin concentrations were measured in culture supernatants after 2-hour incubations. The effective dose reducing methacholine-induced secretion (ED50) was determined. ED50 was 0.25 μM for atropine, 4.10 μM for brompheniramine, and 4.63 μM for chlorpheniramine. None of the anticholinergic drugs changed spontaneous glandular exocytosis. Brompheniramine and chlorpheniramine are equipotent anticholinergic agents in human nasal mucosa in vitro. Atropine was 16 to 19 times more potent.
AB - Brompheniramine and chlorpheniramine have anticholinergic activities, but the relative potency of these effects has not been well defined. The anticholinergic properties of brompheniramine, chlorpheniramine, and atropine were assessed in an in vitro model of human nasal mucosal glandular secretion. Methacholine was used as a cholinergic agonist to stimulate glandular secretion of 7F10-mucin. These drugs (0.01-1000 μM) or vehicle (saline) were added to explant cultures with and without 100 μM methacholine. 7F10-mucin concentrations were measured in culture supernatants after 2-hour incubations. The effective dose reducing methacholine-induced secretion (ED50) was determined. ED50 was 0.25 μM for atropine, 4.10 μM for brompheniramine, and 4.63 μM for chlorpheniramine. None of the anticholinergic drugs changed spontaneous glandular exocytosis. Brompheniramine and chlorpheniramine are equipotent anticholinergic agents in human nasal mucosa in vitro. Atropine was 16 to 19 times more potent.
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U2 - 10.2500/105065898781390271
DO - 10.2500/105065898781390271
M3 - Article
C2 - 9578932
AN - SCOPUS:0031979344
VL - 12
SP - 131
EP - 133
JO - American Journal of Rhinology and Allergy
JF - American Journal of Rhinology and Allergy
SN - 1945-8924
IS - 2
ER -