Antiplatelet effect of sanguinarine is correlated to calcium mobilization, thromboxane and cAMP production

Jiiang Huei Jeng, Hui Lin Wu, Bor Ru Lin, Wan Hong Lan, Hsiao Hwa Chang, Yuan Soon Ho, Po Hsuen Lee, Ying-Jan Wang, Juo Song Wang, Yi Jane Chen, Mei Chi Chang

Research output: Contribution to journalArticle

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Abstract

Sanguinarine is a plant alkaloid present in the root of Sanguinaria canadensis and Poppy fumaria species. Sanguinarine has been used as an antiseptic mouth rinse and a toothpaste additive to reduce dental plaque and gingival inflammation. In this study, we investigated the antiplatelet effects of sanguinarine, aiming to extend its potential pharmacological applications. Sanguinarine inhibited platelet aggregation induced by arachidonic acid (AA), collagen, U46619 and sub-threshold concentration of thrombin (0.05 U/ml) with IC 50 concentrations of 8.3, 7.7, 8.6 and 4.4 μM, respectively. Sanguinarine (5-10 μM) inhibited 10-31% of platelet TXB 2 production, but not platelet aggregation induced by higher concentration of thrombin (0.1 U/ml). SQ29548, a thromboxane receptor antagonist, inhibited the AA-induced platelet aggregation but not TXB 2 production. Sanguinarine suppressed cyclooxygenase-1 (COX-1) activity (IC 50 = 28 μM), whereas its effect on COX-2 activity was minimal. Sanguinarine (8, 10 μM) further inhibited the AA-induced Ca 2+ mobilization by 27-62%. In addition, SQ22536, an adenylate cyclase inhibitor, attenuated the inhibitory effect of sanguinarine toward AA-induced platelet Ca 2+ mobilization and aggregation. These results suggest that sanguinarine is a potent antiplatelet agent, which activates adenylate cyclase, inhibits platelet Ca 2+ mobilization, TXB 2 production as well as suppresses COX-1 enzyme activity. Sanguinarine may have therapeutic potential for treatment of cardiovascular diseases related to platelet aggregation.

Original languageEnglish
Pages (from-to)250-258
Number of pages9
JournalAtherosclerosis
Volume191
Issue number2
DOIs
Publication statusPublished - 2007 Apr 1

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Thromboxanes
Calcium
Platelet Aggregation
Arachidonic Acid
Cyclooxygenase 1
Blood Platelets
Thrombin
Sanguinaria
Fumaria
sanguinarine
Thromboxane Receptors
Papaver
Toothpastes
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Dental Plaque
Local Anti-Infective Agents
Platelet Aggregation Inhibitors
Alkaloids
Adenylyl Cyclases
Mouth

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Jeng, J. H., Wu, H. L., Lin, B. R., Lan, W. H., Chang, H. H., Ho, Y. S., ... Chang, M. C. (2007). Antiplatelet effect of sanguinarine is correlated to calcium mobilization, thromboxane and cAMP production. Atherosclerosis, 191(2), 250-258. https://doi.org/10.1016/j.atherosclerosis.2006.05.023
Jeng, Jiiang Huei ; Wu, Hui Lin ; Lin, Bor Ru ; Lan, Wan Hong ; Chang, Hsiao Hwa ; Ho, Yuan Soon ; Lee, Po Hsuen ; Wang, Ying-Jan ; Wang, Juo Song ; Chen, Yi Jane ; Chang, Mei Chi. / Antiplatelet effect of sanguinarine is correlated to calcium mobilization, thromboxane and cAMP production. In: Atherosclerosis. 2007 ; Vol. 191, No. 2. pp. 250-258.
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abstract = "Sanguinarine is a plant alkaloid present in the root of Sanguinaria canadensis and Poppy fumaria species. Sanguinarine has been used as an antiseptic mouth rinse and a toothpaste additive to reduce dental plaque and gingival inflammation. In this study, we investigated the antiplatelet effects of sanguinarine, aiming to extend its potential pharmacological applications. Sanguinarine inhibited platelet aggregation induced by arachidonic acid (AA), collagen, U46619 and sub-threshold concentration of thrombin (0.05 U/ml) with IC 50 concentrations of 8.3, 7.7, 8.6 and 4.4 μM, respectively. Sanguinarine (5-10 μM) inhibited 10-31{\%} of platelet TXB 2 production, but not platelet aggregation induced by higher concentration of thrombin (0.1 U/ml). SQ29548, a thromboxane receptor antagonist, inhibited the AA-induced platelet aggregation but not TXB 2 production. Sanguinarine suppressed cyclooxygenase-1 (COX-1) activity (IC 50 = 28 μM), whereas its effect on COX-2 activity was minimal. Sanguinarine (8, 10 μM) further inhibited the AA-induced Ca 2+ mobilization by 27-62{\%}. In addition, SQ22536, an adenylate cyclase inhibitor, attenuated the inhibitory effect of sanguinarine toward AA-induced platelet Ca 2+ mobilization and aggregation. These results suggest that sanguinarine is a potent antiplatelet agent, which activates adenylate cyclase, inhibits platelet Ca 2+ mobilization, TXB 2 production as well as suppresses COX-1 enzyme activity. Sanguinarine may have therapeutic potential for treatment of cardiovascular diseases related to platelet aggregation.",
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Jeng, JH, Wu, HL, Lin, BR, Lan, WH, Chang, HH, Ho, YS, Lee, PH, Wang, Y-J, Wang, JS, Chen, YJ & Chang, MC 2007, 'Antiplatelet effect of sanguinarine is correlated to calcium mobilization, thromboxane and cAMP production', Atherosclerosis, vol. 191, no. 2, pp. 250-258. https://doi.org/10.1016/j.atherosclerosis.2006.05.023

Antiplatelet effect of sanguinarine is correlated to calcium mobilization, thromboxane and cAMP production. / Jeng, Jiiang Huei; Wu, Hui Lin; Lin, Bor Ru; Lan, Wan Hong; Chang, Hsiao Hwa; Ho, Yuan Soon; Lee, Po Hsuen; Wang, Ying-Jan; Wang, Juo Song; Chen, Yi Jane; Chang, Mei Chi.

In: Atherosclerosis, Vol. 191, No. 2, 01.04.2007, p. 250-258.

Research output: Contribution to journalArticle

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T1 - Antiplatelet effect of sanguinarine is correlated to calcium mobilization, thromboxane and cAMP production

AU - Jeng, Jiiang Huei

AU - Wu, Hui Lin

AU - Lin, Bor Ru

AU - Lan, Wan Hong

AU - Chang, Hsiao Hwa

AU - Ho, Yuan Soon

AU - Lee, Po Hsuen

AU - Wang, Ying-Jan

AU - Wang, Juo Song

AU - Chen, Yi Jane

AU - Chang, Mei Chi

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Sanguinarine is a plant alkaloid present in the root of Sanguinaria canadensis and Poppy fumaria species. Sanguinarine has been used as an antiseptic mouth rinse and a toothpaste additive to reduce dental plaque and gingival inflammation. In this study, we investigated the antiplatelet effects of sanguinarine, aiming to extend its potential pharmacological applications. Sanguinarine inhibited platelet aggregation induced by arachidonic acid (AA), collagen, U46619 and sub-threshold concentration of thrombin (0.05 U/ml) with IC 50 concentrations of 8.3, 7.7, 8.6 and 4.4 μM, respectively. Sanguinarine (5-10 μM) inhibited 10-31% of platelet TXB 2 production, but not platelet aggregation induced by higher concentration of thrombin (0.1 U/ml). SQ29548, a thromboxane receptor antagonist, inhibited the AA-induced platelet aggregation but not TXB 2 production. Sanguinarine suppressed cyclooxygenase-1 (COX-1) activity (IC 50 = 28 μM), whereas its effect on COX-2 activity was minimal. Sanguinarine (8, 10 μM) further inhibited the AA-induced Ca 2+ mobilization by 27-62%. In addition, SQ22536, an adenylate cyclase inhibitor, attenuated the inhibitory effect of sanguinarine toward AA-induced platelet Ca 2+ mobilization and aggregation. These results suggest that sanguinarine is a potent antiplatelet agent, which activates adenylate cyclase, inhibits platelet Ca 2+ mobilization, TXB 2 production as well as suppresses COX-1 enzyme activity. Sanguinarine may have therapeutic potential for treatment of cardiovascular diseases related to platelet aggregation.

AB - Sanguinarine is a plant alkaloid present in the root of Sanguinaria canadensis and Poppy fumaria species. Sanguinarine has been used as an antiseptic mouth rinse and a toothpaste additive to reduce dental plaque and gingival inflammation. In this study, we investigated the antiplatelet effects of sanguinarine, aiming to extend its potential pharmacological applications. Sanguinarine inhibited platelet aggregation induced by arachidonic acid (AA), collagen, U46619 and sub-threshold concentration of thrombin (0.05 U/ml) with IC 50 concentrations of 8.3, 7.7, 8.6 and 4.4 μM, respectively. Sanguinarine (5-10 μM) inhibited 10-31% of platelet TXB 2 production, but not platelet aggregation induced by higher concentration of thrombin (0.1 U/ml). SQ29548, a thromboxane receptor antagonist, inhibited the AA-induced platelet aggregation but not TXB 2 production. Sanguinarine suppressed cyclooxygenase-1 (COX-1) activity (IC 50 = 28 μM), whereas its effect on COX-2 activity was minimal. Sanguinarine (8, 10 μM) further inhibited the AA-induced Ca 2+ mobilization by 27-62%. In addition, SQ22536, an adenylate cyclase inhibitor, attenuated the inhibitory effect of sanguinarine toward AA-induced platelet Ca 2+ mobilization and aggregation. These results suggest that sanguinarine is a potent antiplatelet agent, which activates adenylate cyclase, inhibits platelet Ca 2+ mobilization, TXB 2 production as well as suppresses COX-1 enzyme activity. Sanguinarine may have therapeutic potential for treatment of cardiovascular diseases related to platelet aggregation.

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