TY - JOUR
T1 - Antiplatelet effects of clausine-D isolated from Clausena excavata
AU - Wu, Chin Chung
AU - Ko, Feng Nien
AU - Wu, Tian Shung
AU - Teng, Che Ming
N1 - Funding Information:
We thank the National Science Council of the Republic of China (NSC82-0420-B002-055-M13) for research support.
PY - 1994/9/28
Y1 - 1994/9/28
N2 - Clausine-D inhibited concentration-dependently the aggregation and release of washed rabbit platelets induced by arachidonic acid and collagen, without affecting those induced by U46619, PAF and thrombin. The IC50 values of clausine-D on arachidonic acid-and collagen-induced platelet aggregation were calculated to be 9.0±1.1 and 58.9±0.9 μM, respectively. Thromboxane B2 and prostaglandin D2 formation in platelets caused by arachidonic acid were also suppressed. Clausine-D inhibited increased intracellular concentration of calcium in platelets caused by arachidonic acid and collagen, and also abolished the generation of inositol monophosphate caused by arachidonic acid, but not that by collagen U46619, PAF and thrombin. In human citrated platelet-rich plasma, clausine-D inhibited the secondary phase, but not the primary phase, of aggregation induced by epinephrine and ADP. These results indicate that the antiplatelet effect of clausine-D is due to inhibition of the formation of thromboxane A2.
AB - Clausine-D inhibited concentration-dependently the aggregation and release of washed rabbit platelets induced by arachidonic acid and collagen, without affecting those induced by U46619, PAF and thrombin. The IC50 values of clausine-D on arachidonic acid-and collagen-induced platelet aggregation were calculated to be 9.0±1.1 and 58.9±0.9 μM, respectively. Thromboxane B2 and prostaglandin D2 formation in platelets caused by arachidonic acid were also suppressed. Clausine-D inhibited increased intracellular concentration of calcium in platelets caused by arachidonic acid and collagen, and also abolished the generation of inositol monophosphate caused by arachidonic acid, but not that by collagen U46619, PAF and thrombin. In human citrated platelet-rich plasma, clausine-D inhibited the secondary phase, but not the primary phase, of aggregation induced by epinephrine and ADP. These results indicate that the antiplatelet effect of clausine-D is due to inhibition of the formation of thromboxane A2.
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U2 - 10.1016/0304-4165(94)90142-2
DO - 10.1016/0304-4165(94)90142-2
M3 - Article
C2 - 7918571
AN - SCOPUS:0028023827
SN - 0304-4165
VL - 1201
SP - 1
EP - 6
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 1
ER -