Antiplatelet effects of protopine isolated from Corydalis tubers

Feng Nien Ko, Tian-Shung Wu, Sheng Teh Lu, Yang Chang Wu, Tur Fu Huang, Che Ming Teng

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Protopine inhibited the aggregation and ATP release of rabbit platelets induced by ADP, arachidonic acid, PAF, collagen and ionophore A23187. Although the platelet aggregation caused by thrombin was not inhibited by protopine (100 ug/ml), the release reaction was partially suppressed. In rabbit platelet-rich plasma, protopine also inhibited the platelet aggregation caused by ADP, arachidonic acid, PAF and collagen. The thromboxane B2 formation of washed platelets caused by arachidonic acid, collagen, ionophore A23187 and thrombin was suppressed by protopine. Protopine inhibited the intracellular calcium increase caused by arachidonic acid in quin-2/AM loaded rabbit platelets. In the presence of indomethacin, the intracellular calcium increase caused by collagen and PAF was completely suppressed by protopine, and the intracellular calcium increase caused by thrombin was partially inhibited. The phosphoinositides breakdown caused by collagen and PAF was inhibited by protopine, but that by thrombin was not affected significantly. Protopine did not cause the elevation of cyclic AMP level of platelets. It is concluded that the antiplatelet effects of protopine is due to inhibition on thromboxane formation and phosphoinositides breakdown and then lead to the decrease of intracellular calcium concentration.

Original languageEnglish
Pages (from-to)289-298
Number of pages10
JournalThrombosis Research
Volume56
Issue number2
DOIs
Publication statusPublished - 1989 Oct 15

All Science Journal Classification (ASJC) codes

  • Hematology

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