TY - JOUR
T1 - Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells
AU - Chen, Ying Hui
AU - Hao, Lyh Jyh
AU - Hung, Chih Peng
AU - Chen, Jung Wei
AU - Leu, Sew Fen
AU - Huang, Bu Miin
N1 - Funding Information:
©The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg 2013 Supported by National Science Council Grant (No.98-2320-B-006-016), Taiwan, China 1. Department of Anesthesia, Chi-Mei Medical Center, Liouying, Tainan, Taiwan, China; 2. Department of Nursing, Min-Hwei College of Health Care Management, Tainan, Taiwan, China; 3. Division of Endocrinology and Metabolism, Kaohsiung Veteran General Hospital Tainan Branch, Tainan, Taiwan, China; 4. Department of Anesthesiology, Chang Gung Memorial Hospital and Kaohsiung Medical Center, Chang Gung University, Kaohsiung, Taiwan, China; 5. Institute of Hot Spring Industry, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, China; 6. Department of Food Science and Technology, College of Human Ecology, HungKuang University, Taichung, Taiwan, China; 7. Department of Cell Biology and Anatomy, and College of Medicine, National Cheng Kung University, Tainan, Taiwan, China Correspondence to: Dr. HUANG Bu-miin, Tel: 886-6-2353535 Ext. 5337, Fax: 886-6-2093007, E-mail: [email protected]. DOI: 10.1007/s11655-013-1453-3
PY - 2014/8
Y1 - 2014/8
N2 - Objective: To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. Methods: The influences of cisplatin (2.5 μg/mL) and/or cordycepin treatment (10 or 100 μmol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Results: Data demonstrated that co-administration of cisplatin (2.5 μg/mL) and cordycepin (10 or 100 μmol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P <0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P <0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P <0.05). Conclusion: Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
AB - Objective: To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. Methods: The influences of cisplatin (2.5 μg/mL) and/or cordycepin treatment (10 or 100 μmol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Results: Data demonstrated that co-administration of cisplatin (2.5 μg/mL) and cordycepin (10 or 100 μmol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P <0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P <0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P <0.05). Conclusion: Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
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U2 - 10.1007/s11655-013-1453-3
DO - 10.1007/s11655-013-1453-3
M3 - Article
C2 - 23543357
AN - SCOPUS:84905561473
SN - 1672-0415
VL - 20
SP - 624
EP - 632
JO - Chinese Journal of Integrative Medicine
JF - Chinese Journal of Integrative Medicine
IS - 8
ER -
