Abstract
A nerve conduit with suitable biomechanical and biochemical environment may improve the regeneration of axons of the injured peripheral nerve. The PC-12 cells were cultured in PRMI medium 1640 and then axonal growth was induced by using nerve growth factor. Time-course morphological changes of the cells were analyzed by using an optical microscope. Regional viscoelasticity and adhesion force of axons were measured by an atomic force microscope by using ramp-and-hold indentation and scratching. The data were fitted with a quasi-viscoelastic model by a method developed previously. Morphological results revealed that cell body length and branch number were highly correlated with axonal growth process and average axon length can be used to define the stage of growth process. Electrical field with intensity of 100mV/mm could enhance the growth rate of axons by 2.88 folds. AFM results showed that the mean elastic modulus of axon increased with stage and the growth cone region has higher Young's modulus than the middle region. Furthermore, the adhesion force on middle region of axon was smaller than that of proximal region and growth cone.
| Original language | English |
|---|---|
| Title of host publication | 13th International Conference on Biomedical Engineering - ICBME 2008 |
| Pages | 1833-1837 |
| Number of pages | 5 |
| DOIs | |
| Publication status | Published - 2009 Dec 1 |
| Event | 13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore Duration: 2008 Dec 3 → 2008 Dec 6 |
Publication series
| Name | IFMBE Proceedings |
|---|---|
| Volume | 23 |
| ISSN (Print) | 1680-0737 |
Other
| Other | 13th International Conference on Biomedical Engineering, ICBME 2008 |
|---|---|
| Country | Singapore |
| Period | 08-12-03 → 08-12-06 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Biomedical Engineering
Cite this
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Application of atomic force microscopy to investigate axonal growth of PC-12 Neuron-like cells. / Ju, Ming-Shaung; Lan, H. M.; Lin, Chou-Ching.
13th International Conference on Biomedical Engineering - ICBME 2008. 2009. p. 1833-1837 (IFMBE Proceedings; Vol. 23).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Application of atomic force microscopy to investigate axonal growth of PC-12 Neuron-like cells
AU - Ju, Ming-Shaung
AU - Lan, H. M.
AU - Lin, Chou-Ching
PY - 2009/12/1
Y1 - 2009/12/1
N2 - A nerve conduit with suitable biomechanical and biochemical environment may improve the regeneration of axons of the injured peripheral nerve. The PC-12 cells were cultured in PRMI medium 1640 and then axonal growth was induced by using nerve growth factor. Time-course morphological changes of the cells were analyzed by using an optical microscope. Regional viscoelasticity and adhesion force of axons were measured by an atomic force microscope by using ramp-and-hold indentation and scratching. The data were fitted with a quasi-viscoelastic model by a method developed previously. Morphological results revealed that cell body length and branch number were highly correlated with axonal growth process and average axon length can be used to define the stage of growth process. Electrical field with intensity of 100mV/mm could enhance the growth rate of axons by 2.88 folds. AFM results showed that the mean elastic modulus of axon increased with stage and the growth cone region has higher Young's modulus than the middle region. Furthermore, the adhesion force on middle region of axon was smaller than that of proximal region and growth cone.
AB - A nerve conduit with suitable biomechanical and biochemical environment may improve the regeneration of axons of the injured peripheral nerve. The PC-12 cells were cultured in PRMI medium 1640 and then axonal growth was induced by using nerve growth factor. Time-course morphological changes of the cells were analyzed by using an optical microscope. Regional viscoelasticity and adhesion force of axons were measured by an atomic force microscope by using ramp-and-hold indentation and scratching. The data were fitted with a quasi-viscoelastic model by a method developed previously. Morphological results revealed that cell body length and branch number were highly correlated with axonal growth process and average axon length can be used to define the stage of growth process. Electrical field with intensity of 100mV/mm could enhance the growth rate of axons by 2.88 folds. AFM results showed that the mean elastic modulus of axon increased with stage and the growth cone region has higher Young's modulus than the middle region. Furthermore, the adhesion force on middle region of axon was smaller than that of proximal region and growth cone.
UR - http://www.scopus.com/inward/record.url?scp=84891941294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891941294&partnerID=8YFLogxK
U2 - 10.1007/978-3-540-92841-6_455
DO - 10.1007/978-3-540-92841-6_455
M3 - Conference contribution
AN - SCOPUS:84891941294
SN - 9783540928409
T3 - IFMBE Proceedings
SP - 1833
EP - 1837
BT - 13th International Conference on Biomedical Engineering - ICBME 2008
ER -