Application of Chitinous materialss in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015

Tzu Wen Liang, Shan Ni Jen, Anh Dzung Nguyen, San Lang Wang

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(L-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalPolymers
Volume8
Issue number3
DOIs
Publication statusPublished - 2016 Mar 22

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Polymers and Plastics

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