Application of microfluidic system to construct cross-linked chitosan microfibers by PDMS chip

Chia Hsien Yeh, Po Wen Lin, Qiaole Zhao, Tsung Che Chou, Yu-Cheng Lin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Polydimethylsiloxane (PDMS) microfluidic chips with various cross-junction angles were designed and fabricated by MEMS and soft lithography technologies to construct chitosan microfibers. Chitosan solution and sodium tripolyphosphate (STPP) solution were injected into the cross-junction microchannel of the microfluidic chip, which generated the laminar flow of chitosan solution by the hydrodynamic focusing. Cross-junction microchannels with angle 30°, 45° and 60° were designed to investigate the generation of laminar flow. The microchannel of angle 30° could produce smaller diameter of chitosan laminar flow than the other angles at the fixed flow rates. Different diameters of laminar flow from 15 μm to 40 μm were successfully generated and transformed into the chitosan microfibers using STPP solution, with the range of chitosan microfiber diameter from 20 μm to 50 μm. The schwann cells and fibroblast cells were employed for cell culture in chitosan microfibers, which would be applied in tissue engineering as scaffold.

Original languageEnglish
Title of host publication4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Pages904-906
Number of pages3
DOIs
Publication statusPublished - 2009 Oct 12
Event4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China
Duration: 2009 Jan 52009 Jan 8

Publication series

Name4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009

Other

Other4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
CountryChina
CityShenzhen
Period09-01-0509-01-08

Fingerprint

Polydimethylsiloxane
Chitosan
Microfluidics
Laminar flow
Microchannels
Sodium
Fibroblasts
Scaffolds (biology)
Tissue engineering
Cell culture
Lithography
MEMS
Hydrodynamics
Cells
Flow rate

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Yeh, C. H., Lin, P. W., Zhao, Q., Chou, T. C., & Lin, Y-C. (2009). Application of microfluidic system to construct cross-linked chitosan microfibers by PDMS chip. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 (pp. 904-906). [5068720] (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068720
Yeh, Chia Hsien ; Lin, Po Wen ; Zhao, Qiaole ; Chou, Tsung Che ; Lin, Yu-Cheng. / Application of microfluidic system to construct cross-linked chitosan microfibers by PDMS chip. 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. pp. 904-906 (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009).
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abstract = "Polydimethylsiloxane (PDMS) microfluidic chips with various cross-junction angles were designed and fabricated by MEMS and soft lithography technologies to construct chitosan microfibers. Chitosan solution and sodium tripolyphosphate (STPP) solution were injected into the cross-junction microchannel of the microfluidic chip, which generated the laminar flow of chitosan solution by the hydrodynamic focusing. Cross-junction microchannels with angle 30°, 45° and 60° were designed to investigate the generation of laminar flow. The microchannel of angle 30° could produce smaller diameter of chitosan laminar flow than the other angles at the fixed flow rates. Different diameters of laminar flow from 15 μm to 40 μm were successfully generated and transformed into the chitosan microfibers using STPP solution, with the range of chitosan microfiber diameter from 20 μm to 50 μm. The schwann cells and fibroblast cells were employed for cell culture in chitosan microfibers, which would be applied in tissue engineering as scaffold.",
author = "Yeh, {Chia Hsien} and Lin, {Po Wen} and Qiaole Zhao and Chou, {Tsung Che} and Yu-Cheng Lin",
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Yeh, CH, Lin, PW, Zhao, Q, Chou, TC & Lin, Y-C 2009, Application of microfluidic system to construct cross-linked chitosan microfibers by PDMS chip. in 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009., 5068720, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, pp. 904-906, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, Shenzhen, China, 09-01-05. https://doi.org/10.1109/NEMS.2009.5068720

Application of microfluidic system to construct cross-linked chitosan microfibers by PDMS chip. / Yeh, Chia Hsien; Lin, Po Wen; Zhao, Qiaole; Chou, Tsung Che; Lin, Yu-Cheng.

4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 904-906 5068720 (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Yeh CH, Lin PW, Zhao Q, Chou TC, Lin Y-C. Application of microfluidic system to construct cross-linked chitosan microfibers by PDMS chip. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 904-906. 5068720. (4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009). https://doi.org/10.1109/NEMS.2009.5068720