Application of virus-induced gene silencing technology in gene functional validation of orchids

Hsiang Chia Lu, Hong-Hwa Chen, Hsin Hung Yeh

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

The largest family of angiosperms, Orchidaceae, has diverse, specialized pollination and ecological strategies and provides a rich subject for investigating evolutionary relationships and developmental biology. However, the study of these non-model organisms may be hindered by challenges, such as their large genome size, low transformation efficiency, long regeneration time, and long life-cycle. To overcome these obstacles, we first developed vectors with the use of a symptomless Cymbidium mosaic virus, which infects most orchids, then combined simple physiological controls and virus-induced gene silencing (VIGS) for validation of gene function in orchids. The success of our strategies was verified by our functional validation of floral identity gene(s) in the tetraploid Phalaenopsis orchids, which have an unusually long life-cycle (two years from sowing to flowering). We could knock down the RNA level of either a specific Phalaenopsis floral identity gene or a family of genes congruently in two months. Functional analysis of orchid genes could become easier and profit from the VIGS approach.

Original languageEnglish
Title of host publicationOrchid Biotechnology
PublisherWorld Scientific Publishing Co.
Pages211-230
Number of pages20
ISBN (Electronic)9789812775900
ISBN (Print)9812706194, 9789812706195
DOIs
Publication statusPublished - 2007 Jan 1

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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