Applications of a rapid and sensitive dengue DUO rapid immunochromatographic test kit as a diagnostic strategy during a dengue type 2 Epidemic in an Urban City

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80-100%), specificity (60-84%), positive predicted value(75%-95%), and negative predicted value (70-100%) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.

Original languageEnglish
Article numbere0158437
JournalPloS one
Volume11
Issue number7
DOIs
Publication statusPublished - 2016 Jul

Fingerprint

analytical kits
dengue
Dengue
Polymerase chain reaction
Immunoglobulin M
Immunoglobulin G
Medical problems
Proteins
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
testing
observational studies
Infection
gold
signs and symptoms (animals and humans)
Observational Studies
Hospital Emergency Service
proteins
Prospective Studies
Health

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

@article{c824fba486984f03bd7ee88013c0e9eb,
title = "Applications of a rapid and sensitive dengue DUO rapid immunochromatographic test kit as a diagnostic strategy during a dengue type 2 Epidemic in an Urban City",
abstract = "Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80-100{\%}), specificity (60-84{\%}), positive predicted value(75{\%}-95{\%}), and negative predicted value (70-100{\%}) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.",
author = "Shih, {Hsin I.} and Hsu, {Hsiang Chin} and Wu, {Chi Jung} and Lin, {Chih Hao} and Chang, {Chia Ming} and Tu, {Yi Fang} and Hsieh, {Chih Chia} and Chi, {Chih Hsien} and Sung, {Tzu Ching}",
year = "2016",
month = "7",
doi = "10.1371/journal.pone.0158437",
language = "English",
volume = "11",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

TY - JOUR

T1 - Applications of a rapid and sensitive dengue DUO rapid immunochromatographic test kit as a diagnostic strategy during a dengue type 2 Epidemic in an Urban City

AU - Shih, Hsin I.

AU - Hsu, Hsiang Chin

AU - Wu, Chi Jung

AU - Lin, Chih Hao

AU - Chang, Chia Ming

AU - Tu, Yi Fang

AU - Hsieh, Chih Chia

AU - Chi, Chih Hsien

AU - Sung, Tzu Ching

PY - 2016/7

Y1 - 2016/7

N2 - Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80-100%), specificity (60-84%), positive predicted value(75%-95%), and negative predicted value (70-100%) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.

AB - Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80-100%), specificity (60-84%), positive predicted value(75%-95%), and negative predicted value (70-100%) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.

UR - http://www.scopus.com/inward/record.url?scp=84978629035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978629035&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0158437

DO - 10.1371/journal.pone.0158437

M3 - Article

C2 - 27415767

AN - SCOPUS:84978629035

VL - 11

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 7

M1 - e0158437

ER -