Applications of protein microarray technology

Sheng Ce Tao, Chien Sheng Chen, Heng Zhu

Research output: Contribution to journalReview article

60 Citations (Scopus)

Abstract

Protein microarrays, an emerging class of proteomic technologies, are quickly becoming essential tools for large-scale and high throughput biochemistry and molecular biology. Recent progress has been made in all the key steps of protein microarray fabrication and application, such as the large-scale cloning of expression-ready prokaryotic and eukaryotic ORFs, high throughput protein purification, surface chemistry, protein delivery systems, and detection methods. Two classes of protein microarrays are currently available: analytical and functional protein microarrays. In the case of analytical protein microarrays, well-characterized molecules with specific activity, such as antibodies, peptide-MHC complexes, or lectins, are used as immobilized probes. These arrays have become one of the most powerful multiplexed detection platforms. Functional protein microarrays are being increasingly applied to many areas of biological discovery, including drug target identification/validation and studies of protein interaction, biochemical activity, and immune responses. Great progress has been achieved in both classes of protein microarrays in terms of sensitivity and specificity, and new protein microarray technologies are continuing to emerge. Finally, protein microarrays have found novel applications in both scientific research and clinical diagnostics.

Original languageEnglish
Pages (from-to)706-718
Number of pages13
JournalCombinatorial Chemistry and High Throughput Screening
Volume10
Issue number8
DOIs
Publication statusPublished - 2007 Sep 1

Fingerprint

Protein Array Analysis
Microarrays
Technology
Proteins
Throughput
Validation Studies
Biochemistry
Molecular biology
Drug Discovery
Cloning
Lectins
Proteomics
Open Reading Frames
Surface chemistry
Organism Cloning
Molecular Biology
Membrane Proteins
Purification
Antibodies
Peptides

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Computer Science Applications
  • Organic Chemistry

Cite this

@article{a840f9764dae4a3a866a904d47aad68a,
title = "Applications of protein microarray technology",
abstract = "Protein microarrays, an emerging class of proteomic technologies, are quickly becoming essential tools for large-scale and high throughput biochemistry and molecular biology. Recent progress has been made in all the key steps of protein microarray fabrication and application, such as the large-scale cloning of expression-ready prokaryotic and eukaryotic ORFs, high throughput protein purification, surface chemistry, protein delivery systems, and detection methods. Two classes of protein microarrays are currently available: analytical and functional protein microarrays. In the case of analytical protein microarrays, well-characterized molecules with specific activity, such as antibodies, peptide-MHC complexes, or lectins, are used as immobilized probes. These arrays have become one of the most powerful multiplexed detection platforms. Functional protein microarrays are being increasingly applied to many areas of biological discovery, including drug target identification/validation and studies of protein interaction, biochemical activity, and immune responses. Great progress has been achieved in both classes of protein microarrays in terms of sensitivity and specificity, and new protein microarray technologies are continuing to emerge. Finally, protein microarrays have found novel applications in both scientific research and clinical diagnostics.",
author = "Tao, {Sheng Ce} and Chen, {Chien Sheng} and Heng Zhu",
year = "2007",
month = "9",
day = "1",
doi = "10.2174/138620707782507386",
language = "English",
volume = "10",
pages = "706--718",
journal = "Combinatorial Chemistry and High Throughput Screening",
issn = "1386-2073",
publisher = "Bentham Science Publishers B.V.",
number = "8",

}

Applications of protein microarray technology. / Tao, Sheng Ce; Chen, Chien Sheng; Zhu, Heng.

In: Combinatorial Chemistry and High Throughput Screening, Vol. 10, No. 8, 01.09.2007, p. 706-718.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Applications of protein microarray technology

AU - Tao, Sheng Ce

AU - Chen, Chien Sheng

AU - Zhu, Heng

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Protein microarrays, an emerging class of proteomic technologies, are quickly becoming essential tools for large-scale and high throughput biochemistry and molecular biology. Recent progress has been made in all the key steps of protein microarray fabrication and application, such as the large-scale cloning of expression-ready prokaryotic and eukaryotic ORFs, high throughput protein purification, surface chemistry, protein delivery systems, and detection methods. Two classes of protein microarrays are currently available: analytical and functional protein microarrays. In the case of analytical protein microarrays, well-characterized molecules with specific activity, such as antibodies, peptide-MHC complexes, or lectins, are used as immobilized probes. These arrays have become one of the most powerful multiplexed detection platforms. Functional protein microarrays are being increasingly applied to many areas of biological discovery, including drug target identification/validation and studies of protein interaction, biochemical activity, and immune responses. Great progress has been achieved in both classes of protein microarrays in terms of sensitivity and specificity, and new protein microarray technologies are continuing to emerge. Finally, protein microarrays have found novel applications in both scientific research and clinical diagnostics.

AB - Protein microarrays, an emerging class of proteomic technologies, are quickly becoming essential tools for large-scale and high throughput biochemistry and molecular biology. Recent progress has been made in all the key steps of protein microarray fabrication and application, such as the large-scale cloning of expression-ready prokaryotic and eukaryotic ORFs, high throughput protein purification, surface chemistry, protein delivery systems, and detection methods. Two classes of protein microarrays are currently available: analytical and functional protein microarrays. In the case of analytical protein microarrays, well-characterized molecules with specific activity, such as antibodies, peptide-MHC complexes, or lectins, are used as immobilized probes. These arrays have become one of the most powerful multiplexed detection platforms. Functional protein microarrays are being increasingly applied to many areas of biological discovery, including drug target identification/validation and studies of protein interaction, biochemical activity, and immune responses. Great progress has been achieved in both classes of protein microarrays in terms of sensitivity and specificity, and new protein microarray technologies are continuing to emerge. Finally, protein microarrays have found novel applications in both scientific research and clinical diagnostics.

UR - http://www.scopus.com/inward/record.url?scp=36049010760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36049010760&partnerID=8YFLogxK

U2 - 10.2174/138620707782507386

DO - 10.2174/138620707782507386

M3 - Review article

C2 - 18045082

AN - SCOPUS:36049010760

VL - 10

SP - 706

EP - 718

JO - Combinatorial Chemistry and High Throughput Screening

JF - Combinatorial Chemistry and High Throughput Screening

SN - 1386-2073

IS - 8

ER -