Aptamer-based sensor for quantitative detection of mercury (II) ions by attenuated total reflection surface enhanced infrared absorption spectroscopy

Chia Wei Liu, Tien Chun Tsai, Masatoshi Osawa, Hsien Chang Chang, Ruey Jen Yang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A sensing platform based on the attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR-SEIRAS) technique and immobilized aptamer has been proposed herein for the selective detection of mercury ions (Hg2+). In the proposed platform, 5′ thiolated 32-mer DNA probes with methylene blue at the 3′ end were immobilized on a thin gold (Au) surface layer. Following Hg2+ ions interacting with T bases of the aptamer, T-Hg-T bonds are formed; resulting in a hairpin-shaped formation of the DNA and a detectable change in the IR absorbance of the sensing interface. Notably, the background noise produced by external molecules (e.g., water, non-specific binding molecules and bulk solution) is reduced to a negligible level by means of the ATR detection mode. It is shown that the proposed sensor has a linear response (R2 = 0.986) with high sensitivity and good selectivity over the Hg2+ range of 0.01 μM–50 μM.

Original languageEnglish
Pages (from-to)137-147
Number of pages11
JournalAnalytica Chimica Acta
Volume1033
DOIs
Publication statusPublished - 2018 Nov 29

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Fingerprint Dive into the research topics of 'Aptamer-based sensor for quantitative detection of mercury (II) ions by attenuated total reflection surface enhanced infrared absorption spectroscopy'. Together they form a unique fingerprint.

  • Cite this