Aptamer probed isolation of circulating tumor cells in cholangiocarcinoma patients

Priya Gopinathan, Nai Jung Chiang, Chih Hung Wang, Anirban Sinha, Yi Cheng Tsai, Hsiu Chi Tu, Shang Cheng Hung, Pang Hung Hsu, Yan Shen Shan, Gwo Bin Lee

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Cholangiocarcinoma (CCA) is a typically lethal, highly metastatic cancer which poses diagnostic challenges and is consequently associated with poor prognosis and high metastatic capability. Since the currently used diagnostic biomarkers are limited in sensitivity and specificity, probes with high-affinity and good specificity are needed to study metastasis in CCA. Circulating tumor cells (CTCs) in blood have been widely accepted as an indicator of diseases progression and metastasis in various other cancers. We hereby report the first use of a CCA specific aptamer probe, coupled on magnetic beads for the isolation of CCA CTCs from 35 advanced stage/metastatic patients on an integrated microfluidic platform with 100 % efficiency (>1 CTC/mL were isolated). The isolated cells were identified via immunofluorescence staining (CK+CD45Hoechst+) and cell counting was assisted by ImageJ. The binding target of the aptamer on the CCA cancer cells was further analyzed and determined to be desmoplakin by liquid chromatography-mass spectrometry, western blotting, and immunofluorescence staining. It is envisioned that this biosensing platform could therefore be used for proactive CCA diagnostics in the near future.

Original languageEnglish
Article number128569
JournalSensors and Actuators, B: Chemical
Volume322
DOIs
Publication statusPublished - 2020 Nov 1

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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