TY - JOUR
T1 - Aqueous extract of Arctium lappa L. root (burdock) enhances chondrogenesis in human bone marrow-derived mesenchymal stem cells
AU - Wu, King Chuen
AU - Weng, Hung Kai
AU - Hsu, Yun Shang
AU - Huang, Pin Jia
AU - Wang, Yang Kao
N1 - Funding Information:
We thank Ms. Yu-Chieh Wang for her excellent technical support and Dr. Yan-Lii Leu (Chang-Gung University) for fingerprint analysis. We thank the English Proofreading Service at Foreign Language Center, National Cheng Kung University for editing the manuscript. The authors are also grateful for support from the Core Research Laboratory, College of Medicine, National Cheng Kung University.
Funding Information:
This work was supported by grants from the Ministry of Science and Technology (MoST 106–2320-B-006-042-MY3), Taiwan R.O.C. to YKW, from National Cheng Kung University Hospital (NCKUH-10804037) Taiwan R.O.C. to HKW, and from Chang Gung Memorial Hospital (CMRPG6H0601) Chia-Yi branch to KCW. The experimental design, data acquisition, data analysis, and data interpretation also used these funds.
Funding Information:
We thank Ms. Yu-Chieh Wang for her excellent technical support and Dr. Yan-Lii Leu (Chang-Gung University) for fingerprint analysis. We thank the English Proofreading Service at Foreign Language Center, National Cheng Kung University for editing the manuscript. The authors are also grateful for support from the Core Research Laboratory, College of Medicine, National Cheng Kung University.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12
Y1 - 2020/12
N2 - Background: Arctium lappa L. root (burdock root) has long been recommended for the treatment of different diseases in traditional Chinese medicine. Burdock root possesses anti-oxidative, anti-inflammatory, anti-cancer, and anti-microbial activities. The aim of the study was to elucidate whether aqueous extract of burdock root regulates mesenchymal stem cell proliferation and differentiation. Methods: Human bone marrow-derived mesenchymal stem cells in 2D high density culture and in 3D micromass pellets were treated with chondrogenic induction medium and chondral basal medium in the absence or presence of aqueous extract of burdock root. The chondrogenic differentiation was accessed by staining glucosaminoglycans, immunostaining SOX9 and type II collagen and immuonblotting of SOX9, aggrecan and type II collagen. Results: Treatment of aqueous extract of burdock root increased the cell proliferation of hMSCs. It did not have significant effect on osteogenic and adipogenic differentiation, but significantly enhanced chondrogenic induction medium-induced chondrogenesis. The increment was dose dependent, as examined by staining glucosaminoglycans, SOX9, and type II collagen and immunobloting of SOX9, aggrecan and type II collagen in 2D and 3D cultures. In the presence of supplemental materials, burdock root aqueous extract showed equivalent chondrogenic induction capability to that of TGF-β. Conclusions: The results demonstrate that aqueous extract of Arctium lappa L. root promotes chondrogenic medium-induced chondrogenic differentiation. The aqueous extract of burdock root can even be used alone to stimulate chondrogenic differentiation. The study suggests that the aqueous extract of burdock root can be used as an alternative strategy for treatment purposes.
AB - Background: Arctium lappa L. root (burdock root) has long been recommended for the treatment of different diseases in traditional Chinese medicine. Burdock root possesses anti-oxidative, anti-inflammatory, anti-cancer, and anti-microbial activities. The aim of the study was to elucidate whether aqueous extract of burdock root regulates mesenchymal stem cell proliferation and differentiation. Methods: Human bone marrow-derived mesenchymal stem cells in 2D high density culture and in 3D micromass pellets were treated with chondrogenic induction medium and chondral basal medium in the absence or presence of aqueous extract of burdock root. The chondrogenic differentiation was accessed by staining glucosaminoglycans, immunostaining SOX9 and type II collagen and immuonblotting of SOX9, aggrecan and type II collagen. Results: Treatment of aqueous extract of burdock root increased the cell proliferation of hMSCs. It did not have significant effect on osteogenic and adipogenic differentiation, but significantly enhanced chondrogenic induction medium-induced chondrogenesis. The increment was dose dependent, as examined by staining glucosaminoglycans, SOX9, and type II collagen and immunobloting of SOX9, aggrecan and type II collagen in 2D and 3D cultures. In the presence of supplemental materials, burdock root aqueous extract showed equivalent chondrogenic induction capability to that of TGF-β. Conclusions: The results demonstrate that aqueous extract of Arctium lappa L. root promotes chondrogenic medium-induced chondrogenic differentiation. The aqueous extract of burdock root can even be used alone to stimulate chondrogenic differentiation. The study suggests that the aqueous extract of burdock root can be used as an alternative strategy for treatment purposes.
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U2 - 10.1186/s12906-020-03158-1
DO - 10.1186/s12906-020-03158-1
M3 - Article
C2 - 33228629
AN - SCOPUS:85096440519
SN - 1472-6882
VL - 20
JO - BMC Complementary Medicine and Therapies
JF - BMC Complementary Medicine and Therapies
IS - 1
M1 - 364
ER -