Aqueous extract of Paeonia suffruticosa inhibits migration and metastasis of renal cell carcinoma cells via suppressing VEGFR-3 pathway

Shih Chin Wang, Sai Wen Tang, Sio-Hong Lam, Chung Chieh Wang, Yu Huei Liu, Hsuan Yuan Lin, Shoei Sheng Lee, Jung Yaw Lin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Renal cell carcinoma (RCC) cells are characterized by strong drug resistance and high metastatic incidence. In this study, the effects of ten kinds of Chinese herbs on RCC cell migration and proliferation were examined. Aqueous extract of Paeonia suffruticosa (PS-A) exerted strong inhibitory effects on cancer cell migration, mobility, and invasion. The results of mouse xenograft experiments showed that the treatment of PS-A significantly suppressed tumor growth and pulmonary metastasis. We further found that PS-A markedly decreased expression of VEGF receptor-3 (VEGFR-3) and phosphorylation of FAK in RCC cells. Moreover, the activation of Rac-1, a modulator of cytoskeletal dynamics, was remarkably reduced by PS-A. Additionally, PS-A suppressed polymerization of actin filament as demonstrated by confocal microscopy analysis and decreased the ratio of F-actin to G-actin in RCC cells, suggesting that PS-A inhibits RCC cell migration through modulating VEGFR-3/FAK/Rac-1 pathway to disrupt actin filament polymerization. In conclusion, this research elucidates the effects and molecular mechanism for antimigration of PS-A on RCC cells and suggests PS-A to be a therapeutic or adjuvant strategy for the patients with aggressive RCC.

Original languageEnglish
Article number409823
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012 Mar 19

Fingerprint

Paeonia
Renal Cell Carcinoma
Neoplasm Metastasis
Cell Movement
Actin Cytoskeleton
Polymerization
Actins
human FLT4 protein
Drug Resistance
Heterografts
Confocal Microscopy
Neoplasms
Phosphorylation
Cell Proliferation
Lung
Incidence
Therapeutics
Growth

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

Cite this

Wang, Shih Chin ; Tang, Sai Wen ; Lam, Sio-Hong ; Wang, Chung Chieh ; Liu, Yu Huei ; Lin, Hsuan Yuan ; Lee, Shoei Sheng ; Lin, Jung Yaw. / Aqueous extract of Paeonia suffruticosa inhibits migration and metastasis of renal cell carcinoma cells via suppressing VEGFR-3 pathway. In: Evidence-based Complementary and Alternative Medicine. 2012 ; Vol. 2012.
@article{32726e948b1544a8a111fd6c0505cc41,
title = "Aqueous extract of Paeonia suffruticosa inhibits migration and metastasis of renal cell carcinoma cells via suppressing VEGFR-3 pathway",
abstract = "Renal cell carcinoma (RCC) cells are characterized by strong drug resistance and high metastatic incidence. In this study, the effects of ten kinds of Chinese herbs on RCC cell migration and proliferation were examined. Aqueous extract of Paeonia suffruticosa (PS-A) exerted strong inhibitory effects on cancer cell migration, mobility, and invasion. The results of mouse xenograft experiments showed that the treatment of PS-A significantly suppressed tumor growth and pulmonary metastasis. We further found that PS-A markedly decreased expression of VEGF receptor-3 (VEGFR-3) and phosphorylation of FAK in RCC cells. Moreover, the activation of Rac-1, a modulator of cytoskeletal dynamics, was remarkably reduced by PS-A. Additionally, PS-A suppressed polymerization of actin filament as demonstrated by confocal microscopy analysis and decreased the ratio of F-actin to G-actin in RCC cells, suggesting that PS-A inhibits RCC cell migration through modulating VEGFR-3/FAK/Rac-1 pathway to disrupt actin filament polymerization. In conclusion, this research elucidates the effects and molecular mechanism for antimigration of PS-A on RCC cells and suggests PS-A to be a therapeutic or adjuvant strategy for the patients with aggressive RCC.",
author = "Wang, {Shih Chin} and Tang, {Sai Wen} and Sio-Hong Lam and Wang, {Chung Chieh} and Liu, {Yu Huei} and Lin, {Hsuan Yuan} and Lee, {Shoei Sheng} and Lin, {Jung Yaw}",
year = "2012",
month = "3",
day = "19",
doi = "10.1155/2012/409823",
language = "English",
volume = "2012",
journal = "Evidence-based Complementary and Alternative Medicine",
issn = "1741-427X",
publisher = "Hindawi Publishing Corporation",

}

Aqueous extract of Paeonia suffruticosa inhibits migration and metastasis of renal cell carcinoma cells via suppressing VEGFR-3 pathway. / Wang, Shih Chin; Tang, Sai Wen; Lam, Sio-Hong; Wang, Chung Chieh; Liu, Yu Huei; Lin, Hsuan Yuan; Lee, Shoei Sheng; Lin, Jung Yaw.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 409823, 19.03.2012.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aqueous extract of Paeonia suffruticosa inhibits migration and metastasis of renal cell carcinoma cells via suppressing VEGFR-3 pathway

AU - Wang, Shih Chin

AU - Tang, Sai Wen

AU - Lam, Sio-Hong

AU - Wang, Chung Chieh

AU - Liu, Yu Huei

AU - Lin, Hsuan Yuan

AU - Lee, Shoei Sheng

AU - Lin, Jung Yaw

PY - 2012/3/19

Y1 - 2012/3/19

N2 - Renal cell carcinoma (RCC) cells are characterized by strong drug resistance and high metastatic incidence. In this study, the effects of ten kinds of Chinese herbs on RCC cell migration and proliferation were examined. Aqueous extract of Paeonia suffruticosa (PS-A) exerted strong inhibitory effects on cancer cell migration, mobility, and invasion. The results of mouse xenograft experiments showed that the treatment of PS-A significantly suppressed tumor growth and pulmonary metastasis. We further found that PS-A markedly decreased expression of VEGF receptor-3 (VEGFR-3) and phosphorylation of FAK in RCC cells. Moreover, the activation of Rac-1, a modulator of cytoskeletal dynamics, was remarkably reduced by PS-A. Additionally, PS-A suppressed polymerization of actin filament as demonstrated by confocal microscopy analysis and decreased the ratio of F-actin to G-actin in RCC cells, suggesting that PS-A inhibits RCC cell migration through modulating VEGFR-3/FAK/Rac-1 pathway to disrupt actin filament polymerization. In conclusion, this research elucidates the effects and molecular mechanism for antimigration of PS-A on RCC cells and suggests PS-A to be a therapeutic or adjuvant strategy for the patients with aggressive RCC.

AB - Renal cell carcinoma (RCC) cells are characterized by strong drug resistance and high metastatic incidence. In this study, the effects of ten kinds of Chinese herbs on RCC cell migration and proliferation were examined. Aqueous extract of Paeonia suffruticosa (PS-A) exerted strong inhibitory effects on cancer cell migration, mobility, and invasion. The results of mouse xenograft experiments showed that the treatment of PS-A significantly suppressed tumor growth and pulmonary metastasis. We further found that PS-A markedly decreased expression of VEGF receptor-3 (VEGFR-3) and phosphorylation of FAK in RCC cells. Moreover, the activation of Rac-1, a modulator of cytoskeletal dynamics, was remarkably reduced by PS-A. Additionally, PS-A suppressed polymerization of actin filament as demonstrated by confocal microscopy analysis and decreased the ratio of F-actin to G-actin in RCC cells, suggesting that PS-A inhibits RCC cell migration through modulating VEGFR-3/FAK/Rac-1 pathway to disrupt actin filament polymerization. In conclusion, this research elucidates the effects and molecular mechanism for antimigration of PS-A on RCC cells and suggests PS-A to be a therapeutic or adjuvant strategy for the patients with aggressive RCC.

UR - http://www.scopus.com/inward/record.url?scp=84863266188&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863266188&partnerID=8YFLogxK

U2 - 10.1155/2012/409823

DO - 10.1155/2012/409823

M3 - Article

VL - 2012

JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

SN - 1741-427X

M1 - 409823

ER -