Aqueous nickel-nitrilotriacetate modified Fe3O 4-NH3+ nanoparticles for protein purification and cell targeting

Dar Bin Shieh, Chia Hao Su, Fong Yu Chang, Ya Na Wu, Wu Chou Su, Jih Ru Hwu, Jyh Horng Chen, Chen Sheng Yeh

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30 Citations (Scopus)

Abstract

A comprehensive totally aqueous phase synthesis of nickel-nitrilotriacetate (Ni-NTA) modified superparamagnetic Fe3O4 nanoparticles is presented. The Fe3O4-NTA-Ni nanoparticles are able to perform efficient and specific purification of 6-His tagged proteins from crude cell lysates, as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The average binding capacity, as demonstrated by streptopain (MW 42kDa), is 0.23mg/mg (protein/Fe3O4-NTA-Ni). Considering the high affinity and specificity of the binding between hexahistidine motif and Ni-NTA, Ni-NTA modified nanoparticles could act as a module to carry 6-His tagged proteins on the particle surface with molecular orientation control, since only the 6-His domain could be attached. These modularly designed functional nanoparticles enhance cancer cell targeting, as supported by the in vitro receptor mediated targeting assay using RGD-4C-6-His fusion peptide. The nanoparticles show no significant hemolysis for human blood and could be investigated further for their in vivo functional imaging applications.

Original languageEnglish
Article number030
Pages (from-to)4174-4182
Number of pages9
JournalNanotechnology
Volume17
Issue number16
DOIs
Publication statusPublished - 2006 Aug 28

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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