TY - JOUR
T1 - Architecture effects of glucose oxidase/Au nanoparticle composite Langmuir-Blodgett films on glucose sensing performance
AU - Wang, Ke Hsuan
AU - Wu, Jau Yann
AU - Chen, Liang Huei
AU - Lee, Yuh Lang
N1 - Funding Information:
This work was sponsored by the National Science Council of Taiwan under grant NSC103-2221-E-006-248-MY3. This research was also supported in part by the Headquarters of University Advancement at National Cheng Kung University, which is sponsored by the Ministry of Education, Taiwan.
Publisher Copyright:
© 2016 Elsevier B.V. All rights reserved.
PY - 2016/3/15
Y1 - 2016/3/15
N2 - The Langmuir-Blodgett (LB) deposition technique is employed to prepare nano-composite films consisting of glucose oxidase (GOx) and gold nanoparticles (AuNPs) for glucose sensing applications. The GOx and AuNPs are co-adsorbed from an aqueous solution onto an air/liquid interface in the presence of an octadecylamine (ODA) template monolayer, forming a mixed (GOx-AuNP) monolayer. Alternatively, a composite film with a cascade architecture (AuNP/GOx) is also prepared by sequentially depositing monolayers of AuNPs and GOx. The architecture effects of the composite LB films on the glucose sensing are studied. The results show that the presence of AuNPs in the co-adsorption system does not affect the adsorption amount and preferred conformation (α-helix) of GOx. Furthermore, the incorporation of AuNPs in both composite films can significantly improve the sensing performance. However, the enhancement effects of the AuNPs in the two architectures are distinct. The major effect of the AuNPs is on the facilitation of charge-transfer in the (GOx-AuNP) film, but on the increase of catalytic activity in the (AuNP/GOx) one. Therefore, the sensing performance can be greatly improved by utilizing a film combining both architectures (AuNP/GOx-AuNP).
AB - The Langmuir-Blodgett (LB) deposition technique is employed to prepare nano-composite films consisting of glucose oxidase (GOx) and gold nanoparticles (AuNPs) for glucose sensing applications. The GOx and AuNPs are co-adsorbed from an aqueous solution onto an air/liquid interface in the presence of an octadecylamine (ODA) template monolayer, forming a mixed (GOx-AuNP) monolayer. Alternatively, a composite film with a cascade architecture (AuNP/GOx) is also prepared by sequentially depositing monolayers of AuNPs and GOx. The architecture effects of the composite LB films on the glucose sensing are studied. The results show that the presence of AuNPs in the co-adsorption system does not affect the adsorption amount and preferred conformation (α-helix) of GOx. Furthermore, the incorporation of AuNPs in both composite films can significantly improve the sensing performance. However, the enhancement effects of the AuNPs in the two architectures are distinct. The major effect of the AuNPs is on the facilitation of charge-transfer in the (GOx-AuNP) film, but on the increase of catalytic activity in the (AuNP/GOx) one. Therefore, the sensing performance can be greatly improved by utilizing a film combining both architectures (AuNP/GOx-AuNP).
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U2 - 10.1016/j.apsusc.2016.01.047
DO - 10.1016/j.apsusc.2016.01.047
M3 - Article
AN - SCOPUS:84959352448
SN - 0169-4332
VL - 366
SP - 202
EP - 209
JO - Applied Surface Science
JF - Applied Surface Science
ER -