Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells

Ying-Jan Wang, Hui Wen Chiu, Yi An Chen, Sheng Yow Ho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.

Original languageEnglish
Title of host publicationUnderstanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress
Subtitle of host publicationArsenic in the Environment
Pages206-208
Number of pages3
Publication statusPublished - 2012 Aug 16
Event4th International Congress on Arsenic in the Environment, As 2012 - Cairns, QLD, Australia
Duration: 2012 Jul 222012 Jul 27

Publication series

NameUnderstanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment

Other

Other4th International Congress on Arsenic in the Environment, As 2012
CountryAustralia
CityCairns, QLD
Period12-07-2212-07-27

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All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Pollution

Cite this

Wang, Y-J., Chiu, H. W., Chen, Y. A., & Ho, S. Y. (2012). Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. In Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment (pp. 206-208). (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).