Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells

Ying-Jan Wang, Hui Wen Chiu, Yi An Chen, Sheng Yow Ho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.

Original languageEnglish
Title of host publicationUnderstanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress
Subtitle of host publicationArsenic in the Environment
Pages206-208
Number of pages3
Publication statusPublished - 2012 Aug 16
Event4th International Congress on Arsenic in the Environment, As 2012 - Cairns, QLD, Australia
Duration: 2012 Jul 222012 Jul 27

Publication series

NameUnderstanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment

Other

Other4th International Congress on Arsenic in the Environment, As 2012
CountryAustralia
CityCairns, QLD
Period12-07-2212-07-27

Fingerprint

androgen
Arsenic
Androgens
arsenic
cancer
Cells
Radiation
Ionizing radiation
Cell death
inhibitor
Cytotoxicity
Phosphatidylinositol 3-Kinases
apoptosis
Tumors
tumor
Apoptosis
arsenic trioxide
radiation

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Pollution

Cite this

Wang, Y-J., Chiu, H. W., Chen, Y. A., & Ho, S. Y. (2012). Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. In Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment (pp. 206-208). (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).
Wang, Ying-Jan ; Chiu, Hui Wen ; Chen, Yi An ; Ho, Sheng Yow. / Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. 2012. pp. 206-208 (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).
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abstract = "LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.",
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Wang, Y-J, Chiu, HW, Chen, YA & Ho, SY 2012, Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. in Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment, pp. 206-208, 4th International Congress on Arsenic in the Environment, As 2012, Cairns, QLD, Australia, 12-07-22.

Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. / Wang, Ying-Jan; Chiu, Hui Wen; Chen, Yi An; Ho, Sheng Yow.

Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. 2012. p. 206-208 (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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PY - 2012/8/16

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N2 - LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.

AB - LNCaP (androgen-sensitive human prostate cancer cells) and PC-3 cells (androgen-independent human prostate cancer cells) were used to investigate the anti-cancer effect of Ionizing Radiation (IR) combined with arsenic trioxide (ATO) and the underlying mechanisms in vitro and in vivo. We found that IR combined with ATO increases the therapeutic efficacy compared to individual treatments in LNCaP and PC-3 cells. Combined treatment induced autophagy and apoptosis in LNCaP cells, and mainly induced autophagy in PC-3 cells. The combined treatment induced cell death was mainly through inhibition of the Akt/mTOR signaling pathways. Furthermore, we found that the combined treatment of cells pre-treated with 3-methyladenine (3-MA) (an autophagy inhibitor) and LY294002 (a specific inhibitor of PI3K) resulted in a significant change in AO-positive cells and cytotoxicity. In in vivo study, the combination treatment possesses anti-tumor growth effect. These novel findings not only suggest a potential therapeutic strategy of the combined treatment for the treatment of androgen-dependent prostate cancer but also in androgen-independent prostate cancer.

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M3 - Conference contribution

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Wang Y-J, Chiu HW, Chen YA, Ho SY. Arsenic trioxide enhances radiation sensitivity of androgen-dependent and -independent human prostate cancer cells. In Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment. 2012. p. 206-208. (Understanding the Geological and Medical Interface of Arsenic, As 2012 - 4th International Congress: Arsenic in the Environment).