Assays for angiotensin converting enzyme inhibitory activity

Bin Wha Chang; Richie L.C. Chen; I. Jen Huang; Hsien Chang Chang

doi:10.1006/abio.2001.5005

Assays for angiotensin converting enzyme inhibitory activity

Bin Wha Chang, Richie L.C. Chen, I. Jen Huang, Hsien Chang Chang

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

A colorimetric method and a capillary electrophoresis procedure were developed for quantifying histidylleucine and hippurate, respectively. The colorimetric method is sensitive (extinction coefficient = 7.5 mM^-1 cm^-1) and reproducible (CV = 1.7%, n = 5), which is based on a selective chromogenic reaction for histidylleucine (λ_max = 390 nm) using o-phthalaldehyde. For samples containing unusually high levels of histidine and/or histidyl peptides, the separation-based approach is preferable. The capillary electrophoresis method makes use of an in-capillary microextraction technique; complicated samples can be measured in less than 4 min without pretreatment. Protocols using both methods to measure angiotensin converting enzyme inhibitory activity were proposed.

Original language	English
Pages (from-to)	84-88
Number of pages	5
Journal	Analytical Biochemistry
Volume	291
Issue number	1
DOIs	https://doi.org/10.1006/abio.2001.5005
Publication status	Published - 2001 Apr 1

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/abio.2001.5005

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title = "Assays for angiotensin converting enzyme inhibitory activity",

abstract = "A colorimetric method and a capillary electrophoresis procedure were developed for quantifying histidylleucine and hippurate, respectively. The colorimetric method is sensitive (extinction coefficient = 7.5 mM-1 cm-1) and reproducible (CV = 1.7%, n = 5), which is based on a selective chromogenic reaction for histidylleucine (λmax = 390 nm) using o-phthalaldehyde. For samples containing unusually high levels of histidine and/or histidyl peptides, the separation-based approach is preferable. The capillary electrophoresis method makes use of an in-capillary microextraction technique; complicated samples can be measured in less than 4 min without pretreatment. Protocols using both methods to measure angiotensin converting enzyme inhibitory activity were proposed.",

author = "Chang, {Bin Wha} and Chen, {Richie L.C.} and Huang, {I. Jen} and Chang, {Hsien Chang}",

note = "Funding Information: The authors thank the National Science Council, Republic of China, for the financial support under Grant NSC 89-2313-B-002-247.",

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AU - Chang, Bin Wha

AU - Chen, Richie L.C.

AU - Huang, I. Jen

AU - Chang, Hsien Chang

N1 - Funding Information: The authors thank the National Science Council, Republic of China, for the financial support under Grant NSC 89-2313-B-002-247.

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N2 - A colorimetric method and a capillary electrophoresis procedure were developed for quantifying histidylleucine and hippurate, respectively. The colorimetric method is sensitive (extinction coefficient = 7.5 mM-1 cm-1) and reproducible (CV = 1.7%, n = 5), which is based on a selective chromogenic reaction for histidylleucine (λmax = 390 nm) using o-phthalaldehyde. For samples containing unusually high levels of histidine and/or histidyl peptides, the separation-based approach is preferable. The capillary electrophoresis method makes use of an in-capillary microextraction technique; complicated samples can be measured in less than 4 min without pretreatment. Protocols using both methods to measure angiotensin converting enzyme inhibitory activity were proposed.

AB - A colorimetric method and a capillary electrophoresis procedure were developed for quantifying histidylleucine and hippurate, respectively. The colorimetric method is sensitive (extinction coefficient = 7.5 mM-1 cm-1) and reproducible (CV = 1.7%, n = 5), which is based on a selective chromogenic reaction for histidylleucine (λmax = 390 nm) using o-phthalaldehyde. For samples containing unusually high levels of histidine and/or histidyl peptides, the separation-based approach is preferable. The capillary electrophoresis method makes use of an in-capillary microextraction technique; complicated samples can be measured in less than 4 min without pretreatment. Protocols using both methods to measure angiotensin converting enzyme inhibitory activity were proposed.

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Assays for angiotensin converting enzyme inhibitory activity

Abstract

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