Assays for angiotensin converting enzyme inhibitory activity

Bin Wha Chang, Richie L.C. Chen, I. Jen Huang, Hsien Chang Chang

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

A colorimetric method and a capillary electrophoresis procedure were developed for quantifying histidylleucine and hippurate, respectively. The colorimetric method is sensitive (extinction coefficient = 7.5 mM-1 cm-1) and reproducible (CV = 1.7%, n = 5), which is based on a selective chromogenic reaction for histidylleucine (λmax = 390 nm) using o-phthalaldehyde. For samples containing unusually high levels of histidine and/or histidyl peptides, the separation-based approach is preferable. The capillary electrophoresis method makes use of an in-capillary microextraction technique; complicated samples can be measured in less than 4 min without pretreatment. Protocols using both methods to measure angiotensin converting enzyme inhibitory activity were proposed.

Original languageEnglish
Pages (from-to)84-88
Number of pages5
JournalAnalytical Biochemistry
Volume291
Issue number1
DOIs
Publication statusPublished - 2001 Apr 1

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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