Assembling Composite Dermal Papilla Spheres with Adipose-derived Stem Cells to Enhance Hair Follicle Induction

Chin Fu Huang, Ya Ju Chang, Yuan-Yu Hsueh, Chia Wei Huang, Duo Hsiang Wang, Tzu Chieh Huang, Yi Ting Wu, Fong-chin Su, Michael Warren Hughes, Cheng Ming Chuong, Chia-Ching Wu

Research output: Contribution to journalArticle

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Abstract

Intradermal adipose tissue plays an essential role for hair follicles (HFs) regeneration by regulating hair cycles. However, the effect of reconstruction of HFs and the involvement of adipose-related cells are poorly understood. We investigated assembly strategies for the interactions of dermal papilla (DP) cells with adipose-derived stem cells (ASCs) in promoting hair formation. DP cells lose DP traits during adherent culture, but preserved DP markers with a unified sphere diameter by seeding on chitosan-coated microenvironments. Next, ASCs isolated from rats were co-cultured with DP spheres by different assembling approaches to determine their interactions; a mixed sphere of ASCs with DP cells (MA-DPS), or a core-shell structure, outer ASCs shell and an inner DP core (CSA-DPS). CSA-DPS exhibited superior DP characteristics compared to MA-DPS. Conditional medium from ASCs, but not differentiated adipocytes, promoted DP markers and functional alkaline phosphatase activity from the DP cells. In vivo patch assay showed the core-shell assembling of CSA-DPS can reconstruct cellular arrangements and microenvironmental niches as dominated by PPARα signal in ASCs to induce the greater hair induction than MA-DPS or DP spheres alone. Therefore, the assembling of a core-shell sphere for DP with ASCs could reconstruct the HF cellular arrangement for hair formation. This paper set the groundwork for further evaluation of the input of other cell types.

Original languageEnglish
Article number26436
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 May 23

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Hair Follicle
Stem Cells
Skin
Hair
Peroxisome Proliferator-Activated Receptors
Chitosan
Adipocytes
Alkaline Phosphatase
Adipose Tissue
Regeneration

All Science Journal Classification (ASJC) codes

  • General

Cite this

Huang, Chin Fu ; Chang, Ya Ju ; Hsueh, Yuan-Yu ; Huang, Chia Wei ; Wang, Duo Hsiang ; Huang, Tzu Chieh ; Wu, Yi Ting ; Su, Fong-chin ; Hughes, Michael Warren ; Chuong, Cheng Ming ; Wu, Chia-Ching. / Assembling Composite Dermal Papilla Spheres with Adipose-derived Stem Cells to Enhance Hair Follicle Induction. In: Scientific reports. 2016 ; Vol. 6.
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abstract = "Intradermal adipose tissue plays an essential role for hair follicles (HFs) regeneration by regulating hair cycles. However, the effect of reconstruction of HFs and the involvement of adipose-related cells are poorly understood. We investigated assembly strategies for the interactions of dermal papilla (DP) cells with adipose-derived stem cells (ASCs) in promoting hair formation. DP cells lose DP traits during adherent culture, but preserved DP markers with a unified sphere diameter by seeding on chitosan-coated microenvironments. Next, ASCs isolated from rats were co-cultured with DP spheres by different assembling approaches to determine their interactions; a mixed sphere of ASCs with DP cells (MA-DPS), or a core-shell structure, outer ASCs shell and an inner DP core (CSA-DPS). CSA-DPS exhibited superior DP characteristics compared to MA-DPS. Conditional medium from ASCs, but not differentiated adipocytes, promoted DP markers and functional alkaline phosphatase activity from the DP cells. In vivo patch assay showed the core-shell assembling of CSA-DPS can reconstruct cellular arrangements and microenvironmental niches as dominated by PPARα signal in ASCs to induce the greater hair induction than MA-DPS or DP spheres alone. Therefore, the assembling of a core-shell sphere for DP with ASCs could reconstruct the HF cellular arrangement for hair formation. This paper set the groundwork for further evaluation of the input of other cell types.",
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Assembling Composite Dermal Papilla Spheres with Adipose-derived Stem Cells to Enhance Hair Follicle Induction. / Huang, Chin Fu; Chang, Ya Ju; Hsueh, Yuan-Yu; Huang, Chia Wei; Wang, Duo Hsiang; Huang, Tzu Chieh; Wu, Yi Ting; Su, Fong-chin; Hughes, Michael Warren; Chuong, Cheng Ming; Wu, Chia-Ching.

In: Scientific reports, Vol. 6, 26436, 23.05.2016.

Research output: Contribution to journalArticle

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AU - Wang, Duo Hsiang

AU - Huang, Tzu Chieh

AU - Wu, Yi Ting

AU - Su, Fong-chin

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