Assessment and characterization of degradation effect for the varied degrees of ultra-violet radiation onto the collagen-bonded polypropylene non-woven fabric surfaces

Yu Chang Tyan, Jiunn Der Liao, Ruth Klauser, Ie Der Wu, Chih Chiang Weng

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Exposure to ultra-violet (UV)-C radiation is a frequently used method to prevent bacteria from invasion of blood-contact biomedical products. Potential damage induced by UV radiation to collagen is of concern due to the decay of bioactivity, considerably correlated with structural alterations. Our current investigation studies the collagen-bonded non-woven polypropylene (PP) fabric surface. In this experiment, antenna-coupling microwave plasma is utilized to activate PP fabric and then the sample is grafted with acrylic acid (AAc). Type III collagen is immobilized by using water soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as coupling agent. The collagen-bonded samples with sample temperature ca. 4°C are then exposed to UV-254nm radiation for different time intervals. By using fourier-transformed infrared with attenuated total reflection (FTIR-ATR) and XPS (X-ray photoelectron spectroscopy), we examine the chemical structures of samples with different treatments. Coomassie brilliant blue G250 method is utilized to quantify the immobilized collagen on the PP fabric surfaces. Blood-clotting effects are evaluated by activated partial thromboplastin time, thrombin time, and fibrinogen concentration tests. By means of cell counter and scanning electron microscopy we count red blood cells and platelets adhesion in the modified porous matrix. Our experimental results have demonstrated that with pAAc-grafting of ca. 173μgcm-2 and immobilized collagen of 80.5±4.7μgcm-2, for human plasma incubated samples of various intervals of UV-254nm radiation, fibrinogen concentration decreases in human plasma, while platelets and red blood cells adhesions increase before UV radiation. However, the required time for thrombination shows significant change for UV radiation exposure of less than 20h (α=0.05). The decay of bioactivity for the UV-irradiated, collagen-bonded surfaces is thus evaluated. Surface analyses indicate that the decrease of R-COOH (derivated from grafted-pAAc or de-carboxylation of collagen), amides degradation (broken-NH), and phenylalanine scission (terminated by -OH, tyrosine formation) may gradually damage collagen by increasing the intervals of UV radiation. These effects considerably influence the bioactivity of the collagen-bonded fabric. The XPS measurements of C 1s core levels at 288.4eV (O=C-NH) and at 289.1eV (O=C-O) illustrate significant decreases of intensity after radiation time ca. 44h. It is clear that UV-254nm radiation exposure for ca. 20h has the potential impact to moderate the bioactivities of collagen and therefore act as a vital factor to accelerate biodegradation.

Original languageEnglish
Pages (from-to)65-76
Number of pages12
JournalBiomaterials
Volume23
Issue number1
DOIs
Publication statusPublished - 2002 Jan 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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