Association of aberrant expression of sex-determining gene fibroblast growth factor 9 with Sertoli cell-only syndrome

Chia Ling Chung, Chun Wun Lu, Yu-Sheng Cheng, Ching Yi Lin, Hsiao-Fang Sun, YungMing Lin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective To investigate the expressions of fibroblast growth factor 9 (FGF9) in normal testes and in testes with Sertoli cell-only syndrome (SCOS), explore the biological function of testicular FGF9, and identify the sequence variants of FGF9 gene in patients with SCOS. Design Retrospective case study. Setting University reproductive clinic. Patient(s) Forty-one patients with SCOS, seven with normal spermatogenesis, and 100 controls. Intervention(s) Protein expressions of testicular FGF9 and sequence variants of FGF9 gene in normal controls and patients with SCOS were studied. The biological function and regulation of testicular FGF9 were assessed in vitro. Main Outcome Measure(s) Expression profiles of testicular FGF9, effects of FGF9 on germ cell proliferation, and sequence variants of the FGF9 gene. Result(s) FGF9 was predominately expressed in the cytoplasm of Leydig cells of normal testis; its expression was significantly decreased in patients with SCOS. Conditioned medium of FGF9-treated Leydig cells stimulated germ cell proliferation. A promoter polymorphism (c.-712C→T) of the FGF9 gene attenuated the promoter activity, which contributes to one of the causes of its low expression. Conclusion(s) In addition to the role of sex determination, FGF9 is expressed in postnatal Leydig cells and is involved in cell-to-cell interaction of testicular function. Aberrant expression of testicular FGF9 is associated with SCOS.

Original languageEnglish
JournalFertility and Sterility
Volume100
Issue number6
DOIs
Publication statusPublished - 2013 Jan 1

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Fibroblast Growth Factor 9
Sertoli Cell-Only Syndrome
Genes
Leydig Cells
Testis
Germ Cells
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

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title = "Association of aberrant expression of sex-determining gene fibroblast growth factor 9 with Sertoli cell-only syndrome",
abstract = "Objective To investigate the expressions of fibroblast growth factor 9 (FGF9) in normal testes and in testes with Sertoli cell-only syndrome (SCOS), explore the biological function of testicular FGF9, and identify the sequence variants of FGF9 gene in patients with SCOS. Design Retrospective case study. Setting University reproductive clinic. Patient(s) Forty-one patients with SCOS, seven with normal spermatogenesis, and 100 controls. Intervention(s) Protein expressions of testicular FGF9 and sequence variants of FGF9 gene in normal controls and patients with SCOS were studied. The biological function and regulation of testicular FGF9 were assessed in vitro. Main Outcome Measure(s) Expression profiles of testicular FGF9, effects of FGF9 on germ cell proliferation, and sequence variants of the FGF9 gene. Result(s) FGF9 was predominately expressed in the cytoplasm of Leydig cells of normal testis; its expression was significantly decreased in patients with SCOS. Conditioned medium of FGF9-treated Leydig cells stimulated germ cell proliferation. A promoter polymorphism (c.-712C→T) of the FGF9 gene attenuated the promoter activity, which contributes to one of the causes of its low expression. Conclusion(s) In addition to the role of sex determination, FGF9 is expressed in postnatal Leydig cells and is involved in cell-to-cell interaction of testicular function. Aberrant expression of testicular FGF9 is associated with SCOS.",
author = "Chung, {Chia Ling} and Lu, {Chun Wun} and Yu-Sheng Cheng and Lin, {Ching Yi} and Hsiao-Fang Sun and YungMing Lin",
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Association of aberrant expression of sex-determining gene fibroblast growth factor 9 with Sertoli cell-only syndrome. / Chung, Chia Ling; Lu, Chun Wun; Cheng, Yu-Sheng; Lin, Ching Yi; Sun, Hsiao-Fang; Lin, YungMing.

In: Fertility and Sterility, Vol. 100, No. 6, 01.01.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Association of aberrant expression of sex-determining gene fibroblast growth factor 9 with Sertoli cell-only syndrome

AU - Chung, Chia Ling

AU - Lu, Chun Wun

AU - Cheng, Yu-Sheng

AU - Lin, Ching Yi

AU - Sun, Hsiao-Fang

AU - Lin, YungMing

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Objective To investigate the expressions of fibroblast growth factor 9 (FGF9) in normal testes and in testes with Sertoli cell-only syndrome (SCOS), explore the biological function of testicular FGF9, and identify the sequence variants of FGF9 gene in patients with SCOS. Design Retrospective case study. Setting University reproductive clinic. Patient(s) Forty-one patients with SCOS, seven with normal spermatogenesis, and 100 controls. Intervention(s) Protein expressions of testicular FGF9 and sequence variants of FGF9 gene in normal controls and patients with SCOS were studied. The biological function and regulation of testicular FGF9 were assessed in vitro. Main Outcome Measure(s) Expression profiles of testicular FGF9, effects of FGF9 on germ cell proliferation, and sequence variants of the FGF9 gene. Result(s) FGF9 was predominately expressed in the cytoplasm of Leydig cells of normal testis; its expression was significantly decreased in patients with SCOS. Conditioned medium of FGF9-treated Leydig cells stimulated germ cell proliferation. A promoter polymorphism (c.-712C→T) of the FGF9 gene attenuated the promoter activity, which contributes to one of the causes of its low expression. Conclusion(s) In addition to the role of sex determination, FGF9 is expressed in postnatal Leydig cells and is involved in cell-to-cell interaction of testicular function. Aberrant expression of testicular FGF9 is associated with SCOS.

AB - Objective To investigate the expressions of fibroblast growth factor 9 (FGF9) in normal testes and in testes with Sertoli cell-only syndrome (SCOS), explore the biological function of testicular FGF9, and identify the sequence variants of FGF9 gene in patients with SCOS. Design Retrospective case study. Setting University reproductive clinic. Patient(s) Forty-one patients with SCOS, seven with normal spermatogenesis, and 100 controls. Intervention(s) Protein expressions of testicular FGF9 and sequence variants of FGF9 gene in normal controls and patients with SCOS were studied. The biological function and regulation of testicular FGF9 were assessed in vitro. Main Outcome Measure(s) Expression profiles of testicular FGF9, effects of FGF9 on germ cell proliferation, and sequence variants of the FGF9 gene. Result(s) FGF9 was predominately expressed in the cytoplasm of Leydig cells of normal testis; its expression was significantly decreased in patients with SCOS. Conditioned medium of FGF9-treated Leydig cells stimulated germ cell proliferation. A promoter polymorphism (c.-712C→T) of the FGF9 gene attenuated the promoter activity, which contributes to one of the causes of its low expression. Conclusion(s) In addition to the role of sex determination, FGF9 is expressed in postnatal Leydig cells and is involved in cell-to-cell interaction of testicular function. Aberrant expression of testicular FGF9 is associated with SCOS.

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