Association of spermatogenic failure with decreased CDC25A expression in infertile men

Yu Sheng Cheng, Pao Lin Kuo, Yen Ni Teng, Ting Yi Kuo, Chia Ling Chung, Ying Hung Lin, Rui Wen Liao, Johnny Shinn Nan Lin, Yung Ming Lin

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Background: DAZ gene family is crucial for human spermatogenesis that requires the precise co-ordination of cell cycle events. CDC25A is recognized as the downstream substrate of DAZ gene family and is thought to function on the M-phase regulation of cell cycles. We investigated the expression profiles of CDC25A in the testes of infertile men and evaluated the relationship between CDC25A levels and testicular phenotype, clinical hormonal parameters and sperm retrieval results. Methods: The protein and mRNA transcript levels of CDC25A in the testes of 40 azoospermic men were determined by immunohistochemistry and quantitative real-time-PCR. CDC25A in human spermatozoa was investigated by western blotting and immunofluorescence staining. Results: The CDC25 A protein was expressed mainly in spermatocyte, spermatid and spermatozoa. CDC25A transcript levels were significantly decreased (P = 0.0009) in patients with spermatogenic failure, especially in men with meiotic arrest and Sertoli cell-only syndrome. Significantly higher CDC25A transcript levels were detected in patients with successful sperm retrieval than in patients with failed sperm retrieval (P = 0.005). Conclusions: Decreased CDC25A is associated with spermatogenic failure and failed sperm retrieval in infertile men. Further studies are necessary to explore the functional roles of CDC25A in human spermatozoa.

Original languageEnglish
Pages (from-to)2346-2352
Number of pages7
JournalHuman Reproduction
Volume21
Issue number9
DOIs
Publication statusPublished - 2006 Sep

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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