Astrocytic CCAAT/Enhancer-binding protein delta contributes to reactive oxygen species formation in neuroinflammation

Shao Ming Wang, Sher Wei Lim, Ya Han Wang, Hong Yi Lin, Ming Derg Lai, Chiung Yuan Ko, Yu-Ming Wang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Excessive reactive oxygen species (ROS) can form an oxidative stress and an associated neuroinflammation. However, the contribution of astrocytes to ROS formation, the cause of the resistance of astrocytes to oxidative stress, and the consequences on neurons remain largely uninvestigated. The transcription factor CCAAT/enhancer-binding protein delta (CEBPD) is highly expressed in astrocytes and has been suggested to contribute to the progress of Alzheimer's disease (AD). In this study, we found that ROS formation and expression of p47 phox and p67 phox , subunits of NADPH oxidase, were increased in AppTg mice but attenuated in AppTg/Cebpd -/- mice. Cebpd can up-regulate p47 phox and p67 phox transcription via a direct binding on their promoters, which results in an increase in intracellular oxidative stress. In addition, Cebpd also up-regulated Cu/Zn superoxide dismutase (Sod1) in astrocytes. Inactivation of Sod1 increased the sensitization to oxidative stress, which provides a reason for the resistance of astrocytes in an oxidative stress environment. Taken together, the study first revealed and dissected the involvement of astrocytic Cebpd in the promotion of oxidative stress and the contribution of CEBPD to the resistance of astrocytes in an oxidative stress environment.

Original languageEnglish
Pages (from-to)104-112
Number of pages9
JournalRedox Biology
Volume16
DOIs
Publication statusPublished - 2018 Jun 1

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CCAAT-Enhancer-Binding Protein-delta
Oxidative stress
Reactive Oxygen Species
Astrocytes
Oxidative Stress
NADPH Oxidase
Transcription
Neurons
Alzheimer Disease
Transcription Factors
Up-Regulation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Cite this

Wang, Shao Ming ; Lim, Sher Wei ; Wang, Ya Han ; Lin, Hong Yi ; Lai, Ming Derg ; Ko, Chiung Yuan ; Wang, Yu-Ming. / Astrocytic CCAAT/Enhancer-binding protein delta contributes to reactive oxygen species formation in neuroinflammation. In: Redox Biology. 2018 ; Vol. 16. pp. 104-112.
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Astrocytic CCAAT/Enhancer-binding protein delta contributes to reactive oxygen species formation in neuroinflammation. / Wang, Shao Ming; Lim, Sher Wei; Wang, Ya Han; Lin, Hong Yi; Lai, Ming Derg; Ko, Chiung Yuan; Wang, Yu-Ming.

In: Redox Biology, Vol. 16, 01.06.2018, p. 104-112.

Research output: Contribution to journalArticle

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AU - Ko, Chiung Yuan

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