TY - JOUR
T1 - Asymmetric expression patterns of brain transthyretin in normal mice and a transgenic mouse model of Alzheimer's disease
AU - Tsai, K. J.
AU - Yang, C. H.
AU - Lee, P. C.
AU - Wang, W. T.
AU - Chiu, M. J.
AU - Shen, C. K.J.
N1 - Funding Information:
We thank Dr. Da-Fu Chen for the discussion with him in the National Taiwan University Hospital. This study was supported by the Academia Sinica, Taipei, Taiwan. K.-J. Tsai is an Academia Sinica Distinguished Postdoctoral Scholar, and C.-K.J. Shen is an Academia Sinica Investigator Awardee.
PY - 2009/3/17
Y1 - 2009/3/17
N2 - Brain asymmetry is linked with several neurological diseases, and transthyretin (TTR) is a protein sequestering β-amyloid (Aβ) and helping to prevent the Alzheimer's disease (AD). We show, by real time reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting, that TTR exhibits a pattern of adult male-specific, leftward distribution in the mouse brain. This asymmetry appeared to be mainly due to the asymmetric distribution of the choroid plexus cells in the ventricles. Unlike the normal mice, however, the hemispheric levels of TTR transcripts of 2- and 6-month-old Tg2576 mice, a transgenic AD mouse model overexpressing Aβ, were symmetric in both sexes. Furthermore, at the age of 10 months when the pathological AD-like features had developed, the level of TTR transcripts in the left hemisphere of the male Tg2576 became significantly lower than the right one. This lowering of TTR transcript is accompanied with a higher Aβ level in the left hemisphere of the 10-month Tg2576 males. Finally, for both genders, the TTR transcript levels in the two hemispheres of aged Tg2576 mice were lower than either the adult Tg2576 or the aged nontransgenic controls. Based on the above, we suggest scenarios to correlate the changes in the levels and hemispheric patterns of TTR expression to the pathogenesis of AD.
AB - Brain asymmetry is linked with several neurological diseases, and transthyretin (TTR) is a protein sequestering β-amyloid (Aβ) and helping to prevent the Alzheimer's disease (AD). We show, by real time reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting, that TTR exhibits a pattern of adult male-specific, leftward distribution in the mouse brain. This asymmetry appeared to be mainly due to the asymmetric distribution of the choroid plexus cells in the ventricles. Unlike the normal mice, however, the hemispheric levels of TTR transcripts of 2- and 6-month-old Tg2576 mice, a transgenic AD mouse model overexpressing Aβ, were symmetric in both sexes. Furthermore, at the age of 10 months when the pathological AD-like features had developed, the level of TTR transcripts in the left hemisphere of the male Tg2576 became significantly lower than the right one. This lowering of TTR transcript is accompanied with a higher Aβ level in the left hemisphere of the 10-month Tg2576 males. Finally, for both genders, the TTR transcript levels in the two hemispheres of aged Tg2576 mice were lower than either the adult Tg2576 or the aged nontransgenic controls. Based on the above, we suggest scenarios to correlate the changes in the levels and hemispheric patterns of TTR expression to the pathogenesis of AD.
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U2 - 10.1016/j.neuroscience.2008.12.045
DO - 10.1016/j.neuroscience.2008.12.045
M3 - Article
C2 - 19167467
AN - SCOPUS:60849134255
SN - 0306-4522
VL - 159
SP - 638
EP - 646
JO - Neuroscience
JF - Neuroscience
IS - 2
ER -