Atherosclerosis induced by arsenic in drinking water in rats through altering lipid metabolism

Tain Junn Cheng, Jiunn Jye Chuu, Chia Yu Chang, Wan Chen Tsai, Kuan Jung Chen, How-Ran Guo

Research output: Contribution to journalArticle

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Abstract

Arsenic in drinking water is a global environmental health problem, and the exposure may increase cardiovascular and cerebrovascular diseases mortalities, most likely through causing atherosclerosis. However, the mechanism of atherosclerosis formation after arsenic exposure is still unclear. To study the mechanism of atherosclerosis formation after arsenic exposure and explore the role of high cholesterol diet (HCD) in this process, we fed spontaneous hypertensive rats and Wistar Kyoto rats with basal diet or HCD and provided with them drinking water containing arsenic at different ages and orders for 20 consecutive weeks. We measured high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), total cholesterol, triglycerides, heat shock protein 70 (HSP 70), and high sensitive C-reactive protein (hs-CRP) at predetermined intervals and determined expressions of cholesteryl ester transfer protein-1 (CETP-1) and liver X receptor β (LXRβ) in the liver. Atherosclerosis was determined by examining the aorta with hematoxylin and eosin stain. After 20. weeks, we found arsenic, alone or combined with HCD, may promote atherosclerosis formation with transient increases in HSP 70 and hs-CRP. Early combination exposure decreased the HDL-C/LDL-C ratio without changing the levels of total cholesterol and triglyceride until 30. weeks old. Both CETP-1 and LXRβ activities were suppressed, most significantly in early combination exposure. In conclusion, arsenic exposure may induce atherosclerosis through modifying reverse cholesterol transport in cholesterol metabolism and suppressing LXRβ and CEPT-1 expressions. For decreasing atherosclerosis related mortality associated with arsenic, preventing exposure from environmental sources in early life is an important element.

Original languageEnglish
Pages (from-to)146-153
Number of pages8
JournalToxicology and Applied Pharmacology
Volume256
Issue number2
DOIs
Publication statusPublished - 2011 Oct 15

Fingerprint

Arsenic
Lipid Metabolism
Drinking Water
Rats
Atherosclerosis
Cholesterol
Nutrition
Liver
Cholesterol Ester Transfer Proteins
Diet
HSP70 Heat-Shock Proteins
C-Reactive Protein
LDL Cholesterol
HDL Cholesterol
Triglycerides
Cerebrovascular Disorders
Inbred WKY Rats
Environmental Health
Mortality
Environmental Exposure

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology

Cite this

Cheng, Tain Junn ; Chuu, Jiunn Jye ; Chang, Chia Yu ; Tsai, Wan Chen ; Chen, Kuan Jung ; Guo, How-Ran. / Atherosclerosis induced by arsenic in drinking water in rats through altering lipid metabolism. In: Toxicology and Applied Pharmacology. 2011 ; Vol. 256, No. 2. pp. 146-153.
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Atherosclerosis induced by arsenic in drinking water in rats through altering lipid metabolism. / Cheng, Tain Junn; Chuu, Jiunn Jye; Chang, Chia Yu; Tsai, Wan Chen; Chen, Kuan Jung; Guo, How-Ran.

In: Toxicology and Applied Pharmacology, Vol. 256, No. 2, 15.10.2011, p. 146-153.

Research output: Contribution to journalArticle

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AU - Chuu, Jiunn Jye

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