Augmented secretion of tumor necrosis factor-alpha during sepsis following splenectomy in mice

T. W. Chang, J. S. Lee, P. W. Lin, M. C. Chang

Research output: Contribution to journalArticle


This research project was purported to investigate the effect of splenectomy on sepsis-induced tumor necrosis factor-alpha (TNF-α) secretion. C3H/HeN mice used in the experiment were subjected to splenectomy (SP group) and sham operation (SS group) three weeks prior to sepsis. Cecal ligation and puncture (CLP) methods were used to produce sepsis. In both splenectomized and sham-splenectomized groups, serum TNF-α levels became detectable by ELISA methods 2 hours after CLP and reached the peak 4 hours (SP group: 380.5 ± 59.3 pg/mL, mean±SEM, n=10; SS group: 117.9 ± 31.8 pg/mL, mean ± SEM, n=10) after CLP. The peak levels of TNF-α concentrations during sepsis in splenectomized group were more than three times higher than that in sham-splenectomized group (p<0.005). In addition, higher survival rates from CLP-induced sepsis were correlated in splenectomized group than in sham-splenectomized group. In summary, these results show that splenectomy is associated with higher serum levels of TNF- α in animals subjected to CLP-induced sepsis, suggesting the negative regulatory effect of the spleen on TNF-α secretion in sepsis. Moreover, higher TNF-α levels after splenectomy may confer protective effect to the host in defending sepsis resulting from CLP.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalJournal of Surgical Association Republic of China
Issue number3
Publication statusPublished - 1997 Jan 1

All Science Journal Classification (ASJC) codes

  • Surgery

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