TY - JOUR
T1 - Autoantibody profiling of monoamine oxidase A knockout mice, an autism spectrum disorder model
AU - Syu, Guan Da
AU - Sutandy, F. X.Reymond
AU - Chen, Kevin
AU - Cheng, Yawei
AU - Chen, Chien Sheng
AU - Shih, Jean C.
N1 - Funding Information:
This work was supported by NIH RO1 MH039085, Tsai family fund and Boyd and Elsie Welin Professorship to J.C. Shih, and the National Science and Technology Council, Taiwan (MOST 108-2410-H-010-005-MY3, MOST 111-2628-B-006 -022 -, MOST 108-2320-B-006 -038 -MY3, NSTC 111-2320-B-006 -041 -MY3, NSTC 111-2622-E-006-042, NSTC 111-2622-8-182A-001-IE), National Yang-Ming University Hospital (RD2019-003), and the Brain Research Center, National Yang-Ming University from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan (108BRC-B501). The study was supported in part by a research grant from the Ministry of Health and Welfare (MOHW111-TDU-B-211-134003) and the Higher Education Sprout Project, Ministry of Education to the Headquarters of University Advancement at National Cheng Kung University (NCKU).
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2023/1
Y1 - 2023/1
N2 - Monoamine oxidase A (MAO A) is the critical enzyme to degrade serotonin in the brain and the knockout mouse exhibits hyperserotonemia and abnormalities that are observed in autism spectrum disorder (ASD). Thus, the MAO A knockout mouse is a valuable model for studying neurological and behavioral impairments in ASD. Based on the immune dysfunction hypothesis, dysregulated humoral immunity may cause neurological impairments. To address this hypothesis, we use high-density proteome microarray to profile the serum antibodies in both wild-type and MAO A knockout mice. The distingue autoantibody signatures were observed in the MAO A knockout and wild-type controls and showed 165 up-regulated and 232 down-regulated autoantibodies. The up-regulated autoantibodies were prone to target brain tissues while down-regulated ones were enriched in sex organs. The identified autoantibodies help bridge the gap between ASD mouse models and humoral immunity, not only yielding insights into the pathological mechanisms but also providing potential biomarkers for translational research in ASD.
AB - Monoamine oxidase A (MAO A) is the critical enzyme to degrade serotonin in the brain and the knockout mouse exhibits hyperserotonemia and abnormalities that are observed in autism spectrum disorder (ASD). Thus, the MAO A knockout mouse is a valuable model for studying neurological and behavioral impairments in ASD. Based on the immune dysfunction hypothesis, dysregulated humoral immunity may cause neurological impairments. To address this hypothesis, we use high-density proteome microarray to profile the serum antibodies in both wild-type and MAO A knockout mice. The distingue autoantibody signatures were observed in the MAO A knockout and wild-type controls and showed 165 up-regulated and 232 down-regulated autoantibodies. The up-regulated autoantibodies were prone to target brain tissues while down-regulated ones were enriched in sex organs. The identified autoantibodies help bridge the gap between ASD mouse models and humoral immunity, not only yielding insights into the pathological mechanisms but also providing potential biomarkers for translational research in ASD.
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U2 - 10.1016/j.bbi.2022.10.001
DO - 10.1016/j.bbi.2022.10.001
M3 - Article
C2 - 36243286
AN - SCOPUS:85140324508
SN - 0889-1591
VL - 107
SP - 193
EP - 200
JO - Brain, Behavior, and Immunity
JF - Brain, Behavior, and Immunity
ER -