Abstract
Thymic interleukin-6 (IL-6), which is produced primarily by thymic epithelial cells, is an important cytokine for T cell growth and differentiation in the thymus. In the present study, we investigated the effects of autocrine IL-6 on the cytokines in primary cultures of human thymic epithelial cells. Using an antibody array and ELISA, in addition to IL-8 and MCP-1, growth-related oncogene-α (GRO-α) was subsequently expressed after IL-6 production. Either IL-6 or GRO-α was time-dependently and strongly expressed in medium with and without serum. Neutralizing IL-6 significantly reduced GRO-α production. Inhibiting Janus kinase 2 (Jak2) spontaneously blocked GRO-α production with or without IL-6 stimulation. We conclude that primary human thymic epithelial cells produce GRO-α and that its expression is regulated primarily by autocrine IL-6 and IL-6-activated Jak2 signaling.
Original language | English |
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Pages (from-to) | 195-201 |
Number of pages | 7 |
Journal | Cytokine |
Volume | 51 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2010 Aug |
All Science Journal Classification (ASJC) codes
- Immunology and Allergy
- Immunology
- Biochemistry
- Molecular Biology
- Hematology