Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform

Priya Gopinathan, Lien Yu Hung, Chih Hung Wang, Nai Jung Chiang, Yu Chun Wang, Yan Shen Shan, Gwo Bin Lee

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

An integrated microfluidic system capable of automatically identifying aptamers specific to cholangiocarcinoma (CCA) cells was developed herein. The developed system was capable of performing cell-based systematic evolution of ligands via an exponential enrichment (Cell-SELEX) process on-chip, and only six rounds of Cell- SELEX were required to identify high specificity aptamers; this represents a significant improvement in speed over conventional SELEX, in which 15-20 rounds are typically required. Using the microfluidic chip developed, three aptamers specific to CCA cells (one for SNU-478 cells and two for HuCCT-1 cells) were successfully screened. This automated system could be modified to uncover aptamer probes against other cancer cells, thereby allowing for earlier diagnosis and consequently a potentially improved prognosis.

Original languageEnglish
Article number044101
JournalBiomicrofluidics
Volume11
Issue number4
DOIs
Publication statusPublished - 2017 Jul 1

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

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