Autophagic machinery activated by dengue virus enhances virus replication

Ying Ray Lee, Huan Yao Lei, Ming Tao Liu, Jen Ren Wang, Shun Hua Chen, Ya Fen Jiang-Shieh, Yee Shin Lin, Trai Ming Yeh, Ching Chuan Liu, Hsiao Sheng Liu

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under the transmission electron microscope. DV2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral replication in the infected cells. Moreover, our data show that ATG5 protein is required to execute DV2-induced autophagy. All together, we are the first to demonstrate that DV can activate autophagic machinery that is favorable for viral replication.

Original languageEnglish
Pages (from-to)240-248
Number of pages9
JournalVirology
Volume374
Issue number2
DOIs
Publication statusPublished - 2008 May 10

All Science Journal Classification (ASJC) codes

  • Virology

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