Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization

Yan Wei Wu, Clément Mettling, Shang Rung Wu, Chia Yi Yu, Guey Chuen Perng, Yee Shin Lin, Yea Lih Lin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

One of the major defense mechanisms against virus spread in vivo is the blocking of viral infectibility by neutralizing antibodies. We describe here the identification of infectious autophagy-associated dengue vesicles released from infected cells. These vesicles contain viral proteins E, NS1, prM/M, and viral RNA, as well as host lipid droplets and LC3-II, an autophagy marker. The viral RNA can be protected within the autophagic organelles since anti-dengue neutralizing antibodies do not have an effect on the vesicle-mediated transmission that is able to initiate a new round of infection in target cells. Importantly, such infectious vesicles were also detected in a patient serum. Our study suggests that autophagy machinery plays a new role in dengue virus transmission. This discovery explains the inefficiency of neutralizing antibody upon dengue infection as a potential immune evasion mechanism in vivo.

Original languageEnglish
Article number32243
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Aug 25

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Dengue
Autophagy
Neutralizing Antibodies
Viral RNA
Antibodies
Immune Evasion
Dengue Virus
Viral Proteins
Infection
Organelles
Viruses
Serum

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "One of the major defense mechanisms against virus spread in vivo is the blocking of viral infectibility by neutralizing antibodies. We describe here the identification of infectious autophagy-associated dengue vesicles released from infected cells. These vesicles contain viral proteins E, NS1, prM/M, and viral RNA, as well as host lipid droplets and LC3-II, an autophagy marker. The viral RNA can be protected within the autophagic organelles since anti-dengue neutralizing antibodies do not have an effect on the vesicle-mediated transmission that is able to initiate a new round of infection in target cells. Importantly, such infectious vesicles were also detected in a patient serum. Our study suggests that autophagy machinery plays a new role in dengue virus transmission. This discovery explains the inefficiency of neutralizing antibody upon dengue infection as a potential immune evasion mechanism in vivo.",
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Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization. / Wu, Yan Wei; Mettling, Clément; Wu, Shang Rung; Yu, Chia Yi; Perng, Guey Chuen; Lin, Yee Shin; Lin, Yea Lih.

In: Scientific reports, Vol. 6, 32243, 25.08.2016.

Research output: Contribution to journalArticle

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