Autophagy induction in T cell-independent acute hepatitis induced by concanavalin A in SCID/NOD mice

C. P. Chang, Huan Yao Lei

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Concanavalin A (Con A) is known to induce acute hepatitis that is mediated by activation of NKT-and T-cell and cytokine production in immunocompetent mice. The observation of Con A-induced autophagic cell death of hepatoma cells via a Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 mediated autophagic pathway made us re-evaluate the effect of Con A-induced hepatitis in mice. Con A was administrated intravenously to BABL/c, SCID, or SCID/NOD mice at doses of 20, 30 or 40 mg/kg, respectively, to induce acute hepatitis. The levels of hepatitis and autophagy induction were both analyzed. We found that Con A can induce acute hepatitis in SCID or SCID/NOD mice with a kinetics similar to that of BALB/c, but requiring a higher dose of Con A. No lymphocyte infiltrations were found in SCID or SCID/NOD mice, and the cytokine productions were different. An autophagy with microtubule-associated protein light chain 3-II conversion was demonstrated in the liver post-Con A injection in SCID/NOD mice. Due to the mannose/ glucose-specific binding on cell membrane, Con A can induce a T-cell-independent acute hepatitis with autophagy in SCID/NOD mice.

Original languageEnglish
Pages (from-to)817-826
Number of pages10
JournalInternational Journal of Immunopathology and Pharmacology
Volume21
Issue number4
DOIs
Publication statusPublished - 2008 Jan 1

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Inbred NOD Mouse
SCID Mice
Autophagy
Concanavalin A
Hepatitis
T-Lymphocytes
Cytokines
Natural Killer T-Cells
Microtubule-Associated Proteins
Mannose
Adenoviridae
Hepatocellular Carcinoma
Cell Membrane
Observation
Lymphocytes
Light
Glucose
Injections
Liver

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

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title = "Autophagy induction in T cell-independent acute hepatitis induced by concanavalin A in SCID/NOD mice",
abstract = "Concanavalin A (Con A) is known to induce acute hepatitis that is mediated by activation of NKT-and T-cell and cytokine production in immunocompetent mice. The observation of Con A-induced autophagic cell death of hepatoma cells via a Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 mediated autophagic pathway made us re-evaluate the effect of Con A-induced hepatitis in mice. Con A was administrated intravenously to BABL/c, SCID, or SCID/NOD mice at doses of 20, 30 or 40 mg/kg, respectively, to induce acute hepatitis. The levels of hepatitis and autophagy induction were both analyzed. We found that Con A can induce acute hepatitis in SCID or SCID/NOD mice with a kinetics similar to that of BALB/c, but requiring a higher dose of Con A. No lymphocyte infiltrations were found in SCID or SCID/NOD mice, and the cytokine productions were different. An autophagy with microtubule-associated protein light chain 3-II conversion was demonstrated in the liver post-Con A injection in SCID/NOD mice. Due to the mannose/ glucose-specific binding on cell membrane, Con A can induce a T-cell-independent acute hepatitis with autophagy in SCID/NOD mice.",
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Autophagy induction in T cell-independent acute hepatitis induced by concanavalin A in SCID/NOD mice. / Chang, C. P.; Lei, Huan Yao.

In: International Journal of Immunopathology and Pharmacology, Vol. 21, No. 4, 01.01.2008, p. 817-826.

Research output: Contribution to journalArticle

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