Autophagy induction in T cell-independent acute hepatitis induced by concanavalin A in SCID/NOD mice

C. P. Chang, Huan Yao Lei

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Concanavalin A (Con A) is known to induce acute hepatitis that is mediated by activation of NKT-and T-cell and cytokine production in immunocompetent mice. The observation of Con A-induced autophagic cell death of hepatoma cells via a Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 mediated autophagic pathway made us re-evaluate the effect of Con A-induced hepatitis in mice. Con A was administrated intravenously to BABL/c, SCID, or SCID/NOD mice at doses of 20, 30 or 40 mg/kg, respectively, to induce acute hepatitis. The levels of hepatitis and autophagy induction were both analyzed. We found that Con A can induce acute hepatitis in SCID or SCID/NOD mice with a kinetics similar to that of BALB/c, but requiring a higher dose of Con A. No lymphocyte infiltrations were found in SCID or SCID/NOD mice, and the cytokine productions were different. An autophagy with microtubule-associated protein light chain 3-II conversion was demonstrated in the liver post-Con A injection in SCID/NOD mice. Due to the mannose/ glucose-specific binding on cell membrane, Con A can induce a T-cell-independent acute hepatitis with autophagy in SCID/NOD mice.

Original languageEnglish
Pages (from-to)817-826
Number of pages10
JournalInternational Journal of Immunopathology and Pharmacology
Volume21
Issue number4
DOIs
Publication statusPublished - 2008 Jan 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Fingerprint Dive into the research topics of 'Autophagy induction in T cell-independent acute hepatitis induced by concanavalin A in SCID/NOD mice'. Together they form a unique fingerprint.

Cite this