Axial-symmetric modeling and kinematic analysis of spreading of sparsely cultured fibroblasts

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Cell spreading plays an important role in the modulation of physiological functions such as inflammation and cancer metastasis. The Brownian ratchet model and Bell's model have been used to simulate actin dynamics and bond kinetics for focal adhesion dynamics, respectively. In the present study, these models were modified and two additional subcellular mechanisms, integrin and myosin kinetics, were incoporated. An integrin recruitment function was introduced to determine the size of a focal adhesion associated with the substrate stiffness. The relationship between myosin concentration and the actin protrusion velocity was described by a first-order differential equation. Subcellular processes, including cell protrusion, focal adhesion formation, and stress fiber formation, were integrated into an axial-symmetric biophysical model, while inputs to the model were kinematic data from time-lapse experiments. Numerical simulations of the model using the Gillespie algorithm showed that dynamics of cell spreading can be well described by the model.

Original languageEnglish
Article number1350062
JournalJournal of Mechanics in Medicine and Biology
Volume13
Issue number4
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

Fibroblasts
Kinematics
Adhesion
Kinetics
Differential equations
Stiffness
Modulation
Fibers
Computer simulation
Substrates
Experiments

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Cite this

@article{777b9f311b604f09a30a32281f2ded4c,
title = "Axial-symmetric modeling and kinematic analysis of spreading of sparsely cultured fibroblasts",
abstract = "Cell spreading plays an important role in the modulation of physiological functions such as inflammation and cancer metastasis. The Brownian ratchet model and Bell's model have been used to simulate actin dynamics and bond kinetics for focal adhesion dynamics, respectively. In the present study, these models were modified and two additional subcellular mechanisms, integrin and myosin kinetics, were incoporated. An integrin recruitment function was introduced to determine the size of a focal adhesion associated with the substrate stiffness. The relationship between myosin concentration and the actin protrusion velocity was described by a first-order differential equation. Subcellular processes, including cell protrusion, focal adhesion formation, and stress fiber formation, were integrated into an axial-symmetric biophysical model, while inputs to the model were kinematic data from time-lapse experiments. Numerical simulations of the model using the Gillespie algorithm showed that dynamics of cell spreading can be well described by the model.",
author = "Wu, {Pei Jung} and Chou-Ching Lin and Ming-Shaung Ju",
year = "2013",
month = "8",
day = "1",
doi = "10.1142/S0219519413500620",
language = "English",
volume = "13",
journal = "Journal of Mechanics in Medicine and Biology",
issn = "0219-5194",
publisher = "World Scientific Publishing Co. Pte Ltd",
number = "4",

}

TY - JOUR

T1 - Axial-symmetric modeling and kinematic analysis of spreading of sparsely cultured fibroblasts

AU - Wu, Pei Jung

AU - Lin, Chou-Ching

AU - Ju, Ming-Shaung

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Cell spreading plays an important role in the modulation of physiological functions such as inflammation and cancer metastasis. The Brownian ratchet model and Bell's model have been used to simulate actin dynamics and bond kinetics for focal adhesion dynamics, respectively. In the present study, these models were modified and two additional subcellular mechanisms, integrin and myosin kinetics, were incoporated. An integrin recruitment function was introduced to determine the size of a focal adhesion associated with the substrate stiffness. The relationship between myosin concentration and the actin protrusion velocity was described by a first-order differential equation. Subcellular processes, including cell protrusion, focal adhesion formation, and stress fiber formation, were integrated into an axial-symmetric biophysical model, while inputs to the model were kinematic data from time-lapse experiments. Numerical simulations of the model using the Gillespie algorithm showed that dynamics of cell spreading can be well described by the model.

AB - Cell spreading plays an important role in the modulation of physiological functions such as inflammation and cancer metastasis. The Brownian ratchet model and Bell's model have been used to simulate actin dynamics and bond kinetics for focal adhesion dynamics, respectively. In the present study, these models were modified and two additional subcellular mechanisms, integrin and myosin kinetics, were incoporated. An integrin recruitment function was introduced to determine the size of a focal adhesion associated with the substrate stiffness. The relationship between myosin concentration and the actin protrusion velocity was described by a first-order differential equation. Subcellular processes, including cell protrusion, focal adhesion formation, and stress fiber formation, were integrated into an axial-symmetric biophysical model, while inputs to the model were kinematic data from time-lapse experiments. Numerical simulations of the model using the Gillespie algorithm showed that dynamics of cell spreading can be well described by the model.

UR - http://www.scopus.com/inward/record.url?scp=84878498139&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878498139&partnerID=8YFLogxK

U2 - 10.1142/S0219519413500620

DO - 10.1142/S0219519413500620

M3 - Article

AN - SCOPUS:84878498139

VL - 13

JO - Journal of Mechanics in Medicine and Biology

JF - Journal of Mechanics in Medicine and Biology

SN - 0219-5194

IS - 4

M1 - 1350062

ER -