Azetidine-induced accumulation of class i small heat shock proteins in the soluble fraction provides thermotolerance in soybean seedlings

Tsung Luo Jinn, Chi Chou Chiu, Wei Wen Song, Yih Ming Chen, Chu Yung Lin

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Abstract

Accumulation of class I small heat shock proteins (sHSPs) is induced by the proline analog, azetidine-2-carboxylic acid (Aze) in soybean seedlings to a level similar to that induced by exposure to 40°C. However, only the treatment with 10 mM Aze for 6 h and subsequently with 10 mM proline for 24 h protected the seedlings from damage during subsequent exposure to 45°C as assessed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. A chaperone activity assay showed that the purified class I sHSPs induced by Aze were functional in vitro and protected proteins from thermal denaturation. Amino acid composition analysis indicated that Aze was not incorporated into de novo synthesized class I sHSPs. Accumulation of class I sHSPs in the soluble post-ribosomal supernatant fraction was found to be important for acquisition of thermotolerance. We suggest that both the accumulation of class I sHSPs and their presence in the soluble fraction are important for establishment of thermotolerance.

Original languageEnglish
Pages (from-to)1759-1767
Number of pages9
JournalPlant and Cell Physiology
Volume45
Issue number12
DOIs
Publication statusPublished - 2004 Dec

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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