Azo dye decolorization with a mutant Escherichia coli strain

Jo-Shu Chang, Tai Shin Kuo, Yun Peng Chao, Jin Yen Ho, Ping Jei Lin

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

A recombinant Escherichia coli strain (E. coli NO3) containing genomic DNA fragments from azo-reducing wild-type Pseudomonas luteola strain decolorized a reactive azo dye (C.I. Reactive Red 22) at approx. 17 mg dye h-1 g cell. The ability to decolorize the azo dye probably did not originate from the plasmid DNA. Acclimation in azo-dye-containing media gave a nearly 10% increase in the decolorization rate of E. coli NO3. Growth with 1.25 g glucose l-1 completely stopped the decolorization activity. When the decolorization metabolites from E. coli NO3 were analyzed by HPLC and MS, the results suggested that decolorization of the azo dye may be due to cleavage of the azo bond.

Original languageEnglish
Pages (from-to)807-812
Number of pages6
JournalBiotechnology Letters
Volume22
Issue number9
DOIs
Publication statusPublished - 2000 Jan 1

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Azo Compounds
Azo dyes
Escherichia coli
DNA
Acclimatization
Metabolites
Pseudomonas
Glucose
Plasmids
Coloring Agents
Dyes
High Pressure Liquid Chromatography
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Chang, Jo-Shu ; Kuo, Tai Shin ; Chao, Yun Peng ; Ho, Jin Yen ; Lin, Ping Jei. / Azo dye decolorization with a mutant Escherichia coli strain. In: Biotechnology Letters. 2000 ; Vol. 22, No. 9. pp. 807-812.
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Azo dye decolorization with a mutant Escherichia coli strain. / Chang, Jo-Shu; Kuo, Tai Shin; Chao, Yun Peng; Ho, Jin Yen; Lin, Ping Jei.

In: Biotechnology Letters, Vol. 22, No. 9, 01.01.2000, p. 807-812.

Research output: Contribution to journalArticle

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