Azo dye decolorization with a mutant Escherichia coli strain

Jo Shu Chang; Tai Shin Kuo; Yun Peng Chao; Jin Yen Ho; Ping Jei Lin

doi:10.1023/A:1005624707777

Azo dye decolorization with a mutant Escherichia coli strain

Jo Shu Chang, Tai Shin Kuo, Yun Peng Chao, Jin Yen Ho, Ping Jei Lin

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81 Citations (Scopus)

Abstract

A recombinant Escherichia coli strain (E. coli NO3) containing genomic DNA fragments from azo-reducing wild-type Pseudomonas luteola strain decolorized a reactive azo dye (C.I. Reactive Red 22) at approx. 17 mg dye h^-1 g cell. The ability to decolorize the azo dye probably did not originate from the plasmid DNA. Acclimation in azo-dye-containing media gave a nearly 10% increase in the decolorization rate of E. coli NO3. Growth with 1.25 g glucose l^-1 completely stopped the decolorization activity. When the decolorization metabolites from E. coli NO3 were analyzed by HPLC and MS, the results suggested that decolorization of the azo dye may be due to cleavage of the azo bond.

Original language	English
Pages (from-to)	807-812
Number of pages	6
Journal	Biotechnology Letters
Volume	22
Issue number	9
DOIs	https://doi.org/10.1023/A:1005624707777
Publication status	Published - 2000

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Azo dye decolorization with a mutant Escherichia coli strain",

abstract = "A recombinant Escherichia coli strain (E. coli NO3) containing genomic DNA fragments from azo-reducing wild-type Pseudomonas luteola strain decolorized a reactive azo dye (C.I. Reactive Red 22) at approx. 17 mg dye h-1 g cell. The ability to decolorize the azo dye probably did not originate from the plasmid DNA. Acclimation in azo-dye-containing media gave a nearly 10% increase in the decolorization rate of E. coli NO3. Growth with 1.25 g glucose l-1 completely stopped the decolorization activity. When the decolorization metabolites from E. coli NO3 were analyzed by HPLC and MS, the results suggested that decolorization of the azo dye may be due to cleavage of the azo bond.",

author = "Chang, {Jo Shu} and Kuo, {Tai Shin} and Chao, {Yun Peng} and Ho, {Jin Yen} and Lin, {Ping Jei}",

note = "Funding Information: The authors gratefully acknowledge the financial support of National Science Council of Republic of China under Grant No. NSC-88-2214-E-035-005. The authors also thank Mr Po-Ting Chen for initiating early experiments of this work.",

year = "2000",

doi = "10.1023/A:1005624707777",

language = "English",

volume = "22",

pages = "807--812",

journal = "Biotechnology Letters",

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publisher = "Springer Netherlands",

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TY - JOUR

T1 - Azo dye decolorization with a mutant Escherichia coli strain

AU - Chang, Jo Shu

AU - Kuo, Tai Shin

AU - Chao, Yun Peng

AU - Ho, Jin Yen

AU - Lin, Ping Jei

N1 - Funding Information: The authors gratefully acknowledge the financial support of National Science Council of Republic of China under Grant No. NSC-88-2214-E-035-005. The authors also thank Mr Po-Ting Chen for initiating early experiments of this work.

PY - 2000

Y1 - 2000

N2 - A recombinant Escherichia coli strain (E. coli NO3) containing genomic DNA fragments from azo-reducing wild-type Pseudomonas luteola strain decolorized a reactive azo dye (C.I. Reactive Red 22) at approx. 17 mg dye h-1 g cell. The ability to decolorize the azo dye probably did not originate from the plasmid DNA. Acclimation in azo-dye-containing media gave a nearly 10% increase in the decolorization rate of E. coli NO3. Growth with 1.25 g glucose l-1 completely stopped the decolorization activity. When the decolorization metabolites from E. coli NO3 were analyzed by HPLC and MS, the results suggested that decolorization of the azo dye may be due to cleavage of the azo bond.

AB - A recombinant Escherichia coli strain (E. coli NO3) containing genomic DNA fragments from azo-reducing wild-type Pseudomonas luteola strain decolorized a reactive azo dye (C.I. Reactive Red 22) at approx. 17 mg dye h-1 g cell. The ability to decolorize the azo dye probably did not originate from the plasmid DNA. Acclimation in azo-dye-containing media gave a nearly 10% increase in the decolorization rate of E. coli NO3. Growth with 1.25 g glucose l-1 completely stopped the decolorization activity. When the decolorization metabolites from E. coli NO3 were analyzed by HPLC and MS, the results suggested that decolorization of the azo dye may be due to cleavage of the azo bond.

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JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 9

ER -

Azo dye decolorization with a mutant Escherichia coli strain

Abstract

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