Azo dye decolorization with a mutant Escherichia coli strain

Jo Shu Chang, Tai Shin Kuo, Yun Peng Chao, Jin Yen Ho, Ping Jei Lin

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81 Citations (Scopus)


A recombinant Escherichia coli strain (E. coli NO3) containing genomic DNA fragments from azo-reducing wild-type Pseudomonas luteola strain decolorized a reactive azo dye (C.I. Reactive Red 22) at approx. 17 mg dye h-1 g cell. The ability to decolorize the azo dye probably did not originate from the plasmid DNA. Acclimation in azo-dye-containing media gave a nearly 10% increase in the decolorization rate of E. coli NO3. Growth with 1.25 g glucose l-1 completely stopped the decolorization activity. When the decolorization metabolites from E. coli NO3 were analyzed by HPLC and MS, the results suggested that decolorization of the azo dye may be due to cleavage of the azo bond.

Original languageEnglish
Pages (from-to)807-812
Number of pages6
JournalBiotechnology Letters
Issue number9
Publication statusPublished - 2000

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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