Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan

Chi Jung Wu, Yin Ching Chuang, Mei Feng Lee, Chin Chi Lee, Hsin Chun Lee, Nan Yao Lee, Chia Ming Chang, Po Lin Chen, Yu Tzu Lin, Jing Jou Yan, Wen Chien Ko

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Although extended-spectrum-β-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combination disk test and the cefepime-clavulanate ESBL Etest. The presence of ESBL-encoding genes, including bla TEM, bla PER, bla CTX-M, and bla SHV genes, was evaluated by PCR and sequence analysis. The results showed that 4 (2.6%) of 156 Aeromonas blood isolates, 1 Aeromonas hydrophila isolate and 3 Aeromonas caviae isolates, expressed an ESBL-producing phenotype. The ESBL gene in two A. caviae isolates was bla PER-3, which was located in both chromosomes and plasmids, as demonstrated by Southern hybridization. Of four patients with ESBL-producing Aeromonas bacteremia, two presented with catheter-related phlebitis and the other two with primary bacteremia. Three patients had been treated with initial noncarbapenem β-lactams for 5 to 10 days, and all survived. In conclusion, ESBL producers exist among Aeromonas blood isolates, and clinical suspicion of ESBL production should be raised in treating infections due to cefotaxime-resistant Aeromonas isolates.

Original languageEnglish
Pages (from-to)5813-5818
Number of pages6
JournalAntimicrobial agents and chemotherapy
Volume55
Issue number12
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

Aeromonas
Bacteremia
Taiwan
Aeromonas caviae
Clavulanic Acid
Genes
Disk Diffusion Antimicrobial Tests
Aeromonas hydrophila
Phenotype
Phlebitis
Lactams
Cefotaxime
Sequence Analysis
Plasmids
Catheters
Chromosomes
Polymerase Chain Reaction
Infection

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Wu, Chi Jung ; Chuang, Yin Ching ; Lee, Mei Feng ; Lee, Chin Chi ; Lee, Hsin Chun ; Lee, Nan Yao ; Chang, Chia Ming ; Chen, Po Lin ; Lin, Yu Tzu ; Yan, Jing Jou ; Ko, Wen Chien. / Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan. In: Antimicrobial agents and chemotherapy. 2011 ; Vol. 55, No. 12. pp. 5813-5818.
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abstract = "Although extended-spectrum-β-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combination disk test and the cefepime-clavulanate ESBL Etest. The presence of ESBL-encoding genes, including bla TEM, bla PER, bla CTX-M, and bla SHV genes, was evaluated by PCR and sequence analysis. The results showed that 4 (2.6{\%}) of 156 Aeromonas blood isolates, 1 Aeromonas hydrophila isolate and 3 Aeromonas caviae isolates, expressed an ESBL-producing phenotype. The ESBL gene in two A. caviae isolates was bla PER-3, which was located in both chromosomes and plasmids, as demonstrated by Southern hybridization. Of four patients with ESBL-producing Aeromonas bacteremia, two presented with catheter-related phlebitis and the other two with primary bacteremia. Three patients had been treated with initial noncarbapenem β-lactams for 5 to 10 days, and all survived. In conclusion, ESBL producers exist among Aeromonas blood isolates, and clinical suspicion of ESBL production should be raised in treating infections due to cefotaxime-resistant Aeromonas isolates.",
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Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan. / Wu, Chi Jung; Chuang, Yin Ching; Lee, Mei Feng; Lee, Chin Chi; Lee, Hsin Chun; Lee, Nan Yao; Chang, Chia Ming; Chen, Po Lin; Lin, Yu Tzu; Yan, Jing Jou; Ko, Wen Chien.

In: Antimicrobial agents and chemotherapy, Vol. 55, No. 12, 01.12.2011, p. 5813-5818.

Research output: Contribution to journalArticle

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T1 - Bacteremia due to extended-spectrum-β-lactamase-producing Aeromonas spp. at a medical center in southern Taiwan

AU - Wu, Chi Jung

AU - Chuang, Yin Ching

AU - Lee, Mei Feng

AU - Lee, Chin Chi

AU - Lee, Hsin Chun

AU - Lee, Nan Yao

AU - Chang, Chia Ming

AU - Chen, Po Lin

AU - Lin, Yu Tzu

AU - Yan, Jing Jou

AU - Ko, Wen Chien

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AB - Although extended-spectrum-β-lactamase (ESBL)-producing aeromonads have been increasingly reported in recent years, most of them were isolates from case reports or environmental isolates. To investigate the prevalence of ESBL producers among Aeromonas blood isolates and the genes encoding ESBLs, consecutive nonduplicate Aeromonas blood isolates collected at a medical center in southern Taiwan from March 2004 to December 2008 were studied. The ESBL phenotypes were examined by clavulanate combination disk test and the cefepime-clavulanate ESBL Etest. The presence of ESBL-encoding genes, including bla TEM, bla PER, bla CTX-M, and bla SHV genes, was evaluated by PCR and sequence analysis. The results showed that 4 (2.6%) of 156 Aeromonas blood isolates, 1 Aeromonas hydrophila isolate and 3 Aeromonas caviae isolates, expressed an ESBL-producing phenotype. The ESBL gene in two A. caviae isolates was bla PER-3, which was located in both chromosomes and plasmids, as demonstrated by Southern hybridization. Of four patients with ESBL-producing Aeromonas bacteremia, two presented with catheter-related phlebitis and the other two with primary bacteremia. Three patients had been treated with initial noncarbapenem β-lactams for 5 to 10 days, and all survived. In conclusion, ESBL producers exist among Aeromonas blood isolates, and clinical suspicion of ESBL production should be raised in treating infections due to cefotaxime-resistant Aeromonas isolates.

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