Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction

Tsung Szu Yeh, Yu-Hua Dean Fang, Chia Hsin Lu, Shao Chieh Chiu, Chia Lin Yeh, Tzu Chen Yen, Yelena Parfyonova, Yu Chen Hu

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Cell sheet technology has been widely employed for the treatment of myocardial infarction (MI), but cell sheet fabrication generally requires the use of thermo-responsive dishes. Here we developed a method for the preparation of adipose-derived stem cell (ASC) sheet that obviated the need of thermo-responsive dishes. This method only required the seeding of rabbit ASC onto 6-well plates at an appropriate cell density and culture in appropriate medium, and the cells were able to develop into ASC sheet in 2 days. The ASC sheetallowed for transduction with the hybrid baculovirus at efficiencies >97%, conferring robust and prolonged (>35 days) overexpression of vascular endothelial growth factor (VEGF). The ASC sheet was easily detached by brief (10s) trypsinization and saline wash, while retaining the extracellular matrix and desired physical properties. The ASC sheet formation and VEGF expression promoted cell survival under hypoxia invitro. Epicardial implantation of the VEGF-expressing ASC sheet to rabbit MI models reduced the infarct size and improved cardiac functions to non-diseased levels, as judged from the left ventrical ejection fraction/myocardial perfusion. The VEGF-expressing ASC sheetalso effectively prevented myocardial wall thinning, suppressed myocardium fibrosis and enhanced blood vessel formation. These data implicated the potential of this method for the preparation of genetically engineered ASC sheet and future MI treatment.

Original languageEnglish
Pages (from-to)174-184
Number of pages11
JournalBiomaterials
Volume35
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Baculoviridae
Stem cells
Infarction
Vascular Endothelial Growth Factor A
Myocardium
Stem Cells
Myocardial Infarction
Rabbits
Intercellular Signaling Peptides and Proteins
Blood vessels
Extracellular Matrix
Blood Vessels
Cell Survival
Fibrosis
Cell Culture Techniques
Perfusion
Physical properties
Cell Count
Cells
Technology

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Yeh, Tsung Szu ; Fang, Yu-Hua Dean ; Lu, Chia Hsin ; Chiu, Shao Chieh ; Yeh, Chia Lin ; Yen, Tzu Chen ; Parfyonova, Yelena ; Hu, Yu Chen. / Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction. In: Biomaterials. 2014 ; Vol. 35, No. 1. pp. 174-184.
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Yeh, TS, Fang, Y-HD, Lu, CH, Chiu, SC, Yeh, CL, Yen, TC, Parfyonova, Y & Hu, YC 2014, 'Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction', Biomaterials, vol. 35, no. 1, pp. 174-184. https://doi.org/10.1016/j.biomaterials.2013.09.080

Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction. / Yeh, Tsung Szu; Fang, Yu-Hua Dean; Lu, Chia Hsin; Chiu, Shao Chieh; Yeh, Chia Lin; Yen, Tzu Chen; Parfyonova, Yelena; Hu, Yu Chen.

In: Biomaterials, Vol. 35, No. 1, 01.01.2014, p. 174-184.

Research output: Contribution to journalArticle

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AU - Yeh, Chia Lin

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AU - Hu, Yu Chen

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