Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia

Kang Yi Lien; Chien Ju Liu; Pao-Lin Kuo; Gwo Bin Lee

doi:10.1109/MEMSYS.2009.4805388

Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia

Kang Yi Lien, Chien Ju Liu, Pao-Lin Kuo, Gwo Bin Lee

Department of Obstetrics Gynecology

Research output: Contribution to journal › Conference article › peer-review

1 Citation (Scopus)

Abstract

This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically.

Original language	English
Article number	4805388
Pages (from-to)	340-343
Number of pages	4
Journal	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
DOIs	https://doi.org/10.1109/MEMSYS.2009.4805388
Publication status	Published - 2009 Jun 1
Event	22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 - Sorrento, Italy Duration: 2009 Jan 25 → 2009 Jan 29

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

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10.1109/MEMSYS.2009.4805388

Cite this

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title = "Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia",

abstract = "This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically.",

author = "Lien, {Kang Yi} and Liu, {Chien Ju} and Pao-Lin Kuo and Lee, {Gwo Bin}",

year = "2009",

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doi = "10.1109/MEMSYS.2009.4805388",

language = "English",

pages = "340--343",

journal = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

issn = "1084-6999",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

note = "22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009 ; Conference date: 25-01-2009 Through 29-01-2009",

}

Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia. / Lien, Kang Yi; Liu, Chien Ju; Kuo, Pao-Lin et al.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 01.06.2009, p. 340-343.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia

AU - Lien, Kang Yi

AU - Liu, Chien Ju

AU - Kuo, Pao-Lin

AU - Lee, Gwo Bin

PY - 2009/6/1

Y1 - 2009/6/1

N2 - This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically.

AB - This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically.

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JO - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

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ER -

Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia

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