Bead-based miniature microfluidic systems for rapid RNA extraction and reverse transcription

Kang Yi Lien, Chien Ju Liu, Jyh Wei Shin, Tsuey Yu Chang, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The current study presents a new integrated microfluidic chip for rapid ribonucleic acid (RNA) purification, extraction and reverse transcription (RT) in an automatic fashion. The miniature system consists of two individual functional devices including a two-way microfluidic control module and a magnetic field/temperature control module. The functional microfluidic control module can perform pumping, mixing, purification and concentration of the RNA samples by incorporating with the magnetic bio-separator consisting of 2-dimension twisted microcoils. Notably, the magnetic bioseparators are developed either to perform the separation of magnetic beads or to control the temperature field for the subsequent RT process. Experimental results showed that the total RNA was successfully purified and extracted by the magnetic beads, and the subsequent RT process of RNA was completed automatically. As a whole, the developed system may provide a powerful platform for biomedical application and biological analysis.

Original languageEnglish
Title of host publication2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008
Pages251-256
Number of pages6
DOIs
Publication statusPublished - 2008 Aug 20
Event1st ASME Micro/Nanoscale Heat Transfer International Conference, MNHT08 - Tainan, Taiwan
Duration: 2008 Jan 62008 Jan 9

Publication series

Name2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008
VolumePART A

Other

Other1st ASME Micro/Nanoscale Heat Transfer International Conference, MNHT08
CountryTaiwan
CityTainan
Period08-01-0608-01-09

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All Science Journal Classification (ASJC) codes

  • Mechanics of Materials
  • Materials Science(all)
  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics

Cite this

Lien, K. Y., Liu, C. J., Shin, J. W., Chang, T. Y., & Lee, G. B. (2008). Bead-based miniature microfluidic systems for rapid RNA extraction and reverse transcription. In 2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008 (pp. 251-256). (2008 Proceedings of the ASME Micro/Nanoscale Heat Transfer International Conference, MNHT 2008; Vol. PART A). https://doi.org/10.1115/MNHT2008-52268