Betanodavirus induces phosphatidylserine exposure and loss of mitochondrial membrane potential in secondary necrotic cells, both of which are blocked by bongkrekic acid

Shi Ping Chen, Huey Lang Yang, Guor Mour Her, Han You Lin, Mei Fen Jeng, Jen Leih Wu, Jiann Ruey Hong

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In this study, we show how the red spotted grouper nervous necrosis virus (RGNNV) causes loss of mitochondrial membrane potential and promotes host secondary apoptotic necrosis. RGNNV viral proteins such as protein α (42 kDa) and protein A (110 kDa) were quickly expressed between 12 h and 24 h postinfection (p.i.) in GL-av cells. Annexin V staining revealed that the NNV infection of GL-av cells induced phosphatidylserine (PS) externalization and development of bulb-like vesicles (bleb formation) at 24 h p.i. NNV infection also induced DNA fragmentation detectable by TUNEL assay between 12 h (8%) and 72 h (32%) p.i. Bongkrekic acid (1.6 μM; BKA) blocked permeability of the mitochondrial permeability transition pore, but cyclosporine A (CsA) did not block secondary necrosis. Finally, secondary necrotic cells were not engulfed by neighboring cells. Our data suggest that RGNNV induces apoptotic death via opening the mitochondrial permeability transition pore thereby triggering secondary necrosis in the mid-apoptotic phase.

Original languageEnglish
Pages (from-to)379-391
Number of pages13
JournalVirology
Volume347
Issue number2
DOIs
Publication statusPublished - 2006 Apr 10

All Science Journal Classification (ASJC) codes

  • Virology

Fingerprint Dive into the research topics of 'Betanodavirus induces phosphatidylserine exposure and loss of mitochondrial membrane potential in secondary necrotic cells, both of which are blocked by bongkrekic acid'. Together they form a unique fingerprint.

  • Cite this