Bile acid and bile acid transporters are involved in the pathogenesis of acute hepatopancreatic necrosis disease in white shrimp Litopenaeus vannamei

Ramya Kumar, Tze Hann Ng, Che Chih Chang, Teng Chun Tung, Shih Shun Lin, Chu Fang Lo, Han Ching Wang

Research output: Contribution to journalArticle

Abstract

Acute hepatopancreas necrosis disease is a recently emerged shrimp disease that is caused by virulent strains of Vibrio parahaemolyticus. Although AHPND poses a serious threat to the shrimp industry, particularly in Asia, its underlying pathogenic mechanisms are not well characterized. Since a previous transcriptomic study showed upregulation of the apical sodium bile acid transporter (LvASBT), our objective here was to explore the role of bile acids and bile acid transporters in AHPND infection. We confirmed that mRNA expression of LvASBT was upregulated in the stomach of AHPND-infected shrimps. Bile acid concentrations were also higher in the stomach of AHPND-infected shrimp and correlated with high expression of pVA plasmid and Pir toxins. In vitro assays showed that bile acids enhanced biofilm formation and increased the release of PirABvp toxins in AHPND-causing V. parahaemolyticus, while in vivo inhibition of LvASBT by GSK2330672 reduced the copy numbers of pVA plasmid, Pir toxin and reduced the amounts of bile acids in AHPND-infected shrimp stomach. Transcriptomics data for AHPND-causing V. parahaemolyticus treated with bile acids showed upregulation of various genes involved in membrane transport, RND efflux pumps and a bacterial secretion system. Taken together, our results show that AHPND-causing V. parahaemolyticus virulence is positively regulated by bile acids and that LvASBT and bile acids in shrimp stomach have important roles in AHPND pathogenesis.

Original languageEnglish
Article numbere13127
JournalCellular Microbiology
Volume22
Issue number1
DOIs
Publication statusPublished - 2020 Jan 1

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Bile Acids and Salts
Necrosis
Vibrio parahaemolyticus
Stomach
Bacterial Secretion Systems
Plasmids
Up-Regulation
Hepatopancreas
Biofilms
bile acid binding proteins
Virulence
Industry
Sodium
Messenger RNA
Membranes
Infection
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

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title = "Bile acid and bile acid transporters are involved in the pathogenesis of acute hepatopancreatic necrosis disease in white shrimp Litopenaeus vannamei",
abstract = "Acute hepatopancreas necrosis disease is a recently emerged shrimp disease that is caused by virulent strains of Vibrio parahaemolyticus. Although AHPND poses a serious threat to the shrimp industry, particularly in Asia, its underlying pathogenic mechanisms are not well characterized. Since a previous transcriptomic study showed upregulation of the apical sodium bile acid transporter (LvASBT), our objective here was to explore the role of bile acids and bile acid transporters in AHPND infection. We confirmed that mRNA expression of LvASBT was upregulated in the stomach of AHPND-infected shrimps. Bile acid concentrations were also higher in the stomach of AHPND-infected shrimp and correlated with high expression of pVA plasmid and Pir toxins. In vitro assays showed that bile acids enhanced biofilm formation and increased the release of PirABvp toxins in AHPND-causing V. parahaemolyticus, while in vivo inhibition of LvASBT by GSK2330672 reduced the copy numbers of pVA plasmid, Pir toxin and reduced the amounts of bile acids in AHPND-infected shrimp stomach. Transcriptomics data for AHPND-causing V. parahaemolyticus treated with bile acids showed upregulation of various genes involved in membrane transport, RND efflux pumps and a bacterial secretion system. Taken together, our results show that AHPND-causing V. parahaemolyticus virulence is positively regulated by bile acids and that LvASBT and bile acids in shrimp stomach have important roles in AHPND pathogenesis.",
author = "Ramya Kumar and Ng, {Tze Hann} and Chang, {Che Chih} and Tung, {Teng Chun} and Lin, {Shih Shun} and Lo, {Chu Fang} and Wang, {Han Ching}",
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Bile acid and bile acid transporters are involved in the pathogenesis of acute hepatopancreatic necrosis disease in white shrimp Litopenaeus vannamei. / Kumar, Ramya; Ng, Tze Hann; Chang, Che Chih; Tung, Teng Chun; Lin, Shih Shun; Lo, Chu Fang; Wang, Han Ching.

In: Cellular Microbiology, Vol. 22, No. 1, e13127, 01.01.2020.

Research output: Contribution to journalArticle

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T1 - Bile acid and bile acid transporters are involved in the pathogenesis of acute hepatopancreatic necrosis disease in white shrimp Litopenaeus vannamei

AU - Kumar, Ramya

AU - Ng, Tze Hann

AU - Chang, Che Chih

AU - Tung, Teng Chun

AU - Lin, Shih Shun

AU - Lo, Chu Fang

AU - Wang, Han Ching

PY - 2020/1/1

Y1 - 2020/1/1

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