Binding specificity of α-bilirubin-imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate) toward α-bilirubin

Mei-Jywan Syu, Jing Hong Deng, You Ming Nian, Tsung Chieh Chiu, An Hua Wu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

α-Bilirubin is an important index to determine the liver's functions. Poly(methacrylic acid-co-ethylene glycol dimethylacrylate) (poly(MAA-co-EGDMA)) imprinted with α-bilirubin was proposed and shown to be able to bind α-bilirubin specifically. The extraction condition was also discussed. Polymers prepared by imprinting bilirubin in poly(MAA-co-EGDMA) and in poly(β-cyclodextrin-co-EGDMA) were compared. In this work, binding specificity of molecularly imprinted polymer (MIP) toward the target template, α-bilirubin, is discussed. Adsorption profile of α-bilirubin by bilirubin-imprinted poly(MAA-co-EGDMA) was measured as a function of time, from where the equilibrium could be determined. Two hours was determined to be the proper time for adsorption. Biliverdin as a rather similar analog compound of bilirubin was chosen for comparison of binding specificity in this study. Progesterone as well as testosterone was also chosen for study because they both co-exist in serum with bilirubin and might interfere with binding capacity of imprinted poly(MAA-co-EGDMA) toward α-bilirubin. Specificity of this polymer for bilirubin was thus confirmed by tasks carried out in mixture solutions comprised of compounds above. It is true that biliverdin contained in the binary mixture did affect the binding capacity of bilirubin. Nevertheless, polymer's binding specificity for bilirubin was essentially sufficient for recognition of α-bilirubin in the presence of other compounds. How MIP identified the target template molecule, α-bilirubin, is also elucidated.

Original languageEnglish
Pages (from-to)4684-4692
Number of pages9
JournalBiomaterials
Volume26
Issue number22
DOIs
Publication statusPublished - 2005 Aug 1

Fingerprint

Ethylene Glycol
Ethylene glycol
Bilirubin
Acids
Polymers
Adsorption
Cyclodextrins
Binary mixtures
Liver
Biliverdine
methacrylic acid
poly(methacrylic acid-ethylene glycol)
Molecules
Progesterone

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Syu, Mei-Jywan ; Deng, Jing Hong ; Nian, You Ming ; Chiu, Tsung Chieh ; Wu, An Hua. / Binding specificity of α-bilirubin-imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate) toward α-bilirubin. In: Biomaterials. 2005 ; Vol. 26, No. 22. pp. 4684-4692.
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Binding specificity of α-bilirubin-imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate) toward α-bilirubin. / Syu, Mei-Jywan; Deng, Jing Hong; Nian, You Ming; Chiu, Tsung Chieh; Wu, An Hua.

In: Biomaterials, Vol. 26, No. 22, 01.08.2005, p. 4684-4692.

Research output: Contribution to journalArticle

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